Platelet-Rich Fibrin–Augmented Gap-Bridging Strategy in Rabbit Anterior Cruciate Ligament Repair: Response

Author’s Response:First of all, we would like to thank Professor Moura ̃oand Professor Lowenstein for providing constructive com-ments and suggestions regarding the blood collection andanalytic method of rabbit platelet-rich fibrin (PRF) in ourrecent article published inAJSM.To prepare rabbit’s PRF, the animals were first put intoan acrylic restraint box and their ear’s marginal veins wereexposed. A 23-gauge scalp vein set was then carefullyinserted and blood was collected. Occasionally, only 4 mLof blood could be collected, and we would pool 2 tubes ofblood together before sending the blood for centrifugation.We do find that the blood flow from the ear’s vein is slow,and sometimes failure to draw sufficient blood or clot for-mation may occur. Therefore, we are now developinga new manner of blood collection by drawing blood fromthe jugular vein through an ultrasound-guided method tofurther improve the efficiency of preparing PRF.