Platelet Extracellular Vesicles Loaded Gelatine Hydrogels for Wound Care

Florence Back, Alexandre Barras, Ariunjargal Nyam-Erdene, Jen Chang Yang, Sorin Melinte, José Rumipamba, Thierry Burnouf, Rabah Boukherroub, Sabine Szunerits, Er Yuan Chuang

研究成果: 雜誌貢獻文章同行評審

1 引文 斯高帕斯(Scopus)

摘要

Platelet extracellular vesicles (pEVs) isolated from clinical-grade human platelet concentrates are attracting attention as a promising agent for wound healing therapies. Although pEVs have shown potential for skin regeneration, their incorporation into wound bandages has remained limitedly explored. Herein, gelatine-based hydrogel (PAH-G) foams for pEVs loading and release are formulated by crosslinking gelatine with poly(allylamine) hydrochloride (PAH) in the presence of glutaraldehyde and sodium bicarbonate. The optimized PAH-G hydrogel foam, PAH0.24G37, displayed an elastic modulus G’ = 8.5 kPa at 37 °C and retained a rubbery state at elevated temperatures. The excellent swelling properties of PAH0.24G37 allowed to easily absorb pEVs at high concentration (1 × 1011 particles mL−1). The therapeutic effect of pEVs was evaluated in vivo on a chronic wound rat model. These studies demonstrated full wound closure after 14 days upon treatment with PAH0.24G37@pEVs. The maintenance of a reduced-inflammatory environment from the onset of treatment promoted a quicker transition to skin remodeling. Promotion of follicle activation and angiogenesis as well as M1–M2 macrophage modulation are evidenced. Altogether, the multifunctional properties of PAH0.24G37@pEVs addressed the complex challenges associated with chronic diabetic wounds, representing a significant advance toward personalized treatment regimens for these conditions.
原文英語
文章編號2401914
期刊Advanced healthcare materials
14
發行號1
DOIs
出版狀態接受/付印 - 2024

ASJC Scopus subject areas

  • 生物材料
  • 生物醫學工程
  • 藥學科學

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