TY - JOUR
T1 - Physicochemical and antioxidative characteristics of potato protein isolate hydrolysate
AU - Chang, Chiung Yueh
AU - Jin, Jinn Der
AU - Chang, Hsiao Li
AU - Huang, Ko Chieh
AU - Chiang, Yi Fen
AU - Hsia, Shih Min
N1 - Funding Information:
Funding: This research was funded from the Ministry of Science and Technology (MOST; Taiwan, Republic of China): the grants number (MOST109-2628-B-038-015-, 109-2314-B-038-059-and 106-2320-B-038-064-MY3).
Publisher Copyright:
© 2020 by the authors.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/10
Y1 - 2020/10
N2 - This study investigated the physicochemical characteristics of potato protein isolate hydrolysate (PPIH) and its antioxidant activity. Potato protein isolate (PPI) was hydrolyzed into PPIH by the proteases bromelain, Neutrase, and Flavourzyme. Compared with PPI, the resulting PPIH had a lower molecular weight (MW, from 103.5 to 422.7 Da) and smaller particle size (<50 nm), as well as a higher solubility rate (>70%) under acidic conditions (pH 3-6). PPIH presented good solubility (73%) across the tested pH range of 3-6. As the pH was increased, the zeta potential of PPIH decreased from -7.4 to -21.6. Using the 2,20-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid (ABTS) radical-scavenging assay, we determined that the half-maximal effective concentration (EC50) values of ascorbic acid, PPIH, and PPI were 0.01, 0.89, and >2.33 mg/mL, respectively. Furthermore, PPIH (50 μg/mL) protected C2C12 cells fromH2O2 oxidation significantly better than PPI (10.5% higher viability rate; p < 0.01). These findings demonstrated the possible use of PPIH as an antioxidant in medical applications.
AB - This study investigated the physicochemical characteristics of potato protein isolate hydrolysate (PPIH) and its antioxidant activity. Potato protein isolate (PPI) was hydrolyzed into PPIH by the proteases bromelain, Neutrase, and Flavourzyme. Compared with PPI, the resulting PPIH had a lower molecular weight (MW, from 103.5 to 422.7 Da) and smaller particle size (<50 nm), as well as a higher solubility rate (>70%) under acidic conditions (pH 3-6). PPIH presented good solubility (73%) across the tested pH range of 3-6. As the pH was increased, the zeta potential of PPIH decreased from -7.4 to -21.6. Using the 2,20-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid (ABTS) radical-scavenging assay, we determined that the half-maximal effective concentration (EC50) values of ascorbic acid, PPIH, and PPI were 0.01, 0.89, and >2.33 mg/mL, respectively. Furthermore, PPIH (50 μg/mL) protected C2C12 cells fromH2O2 oxidation significantly better than PPI (10.5% higher viability rate; p < 0.01). These findings demonstrated the possible use of PPIH as an antioxidant in medical applications.
KW - Antioxidant
KW - Hydrolysate
KW - Physicochemical characteristics
KW - Potato protein isolate
KW - Solubility
UR - http://www.scopus.com/inward/record.url?scp=85092447796&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85092447796&partnerID=8YFLogxK
U2 - 10.3390/molecules25194450
DO - 10.3390/molecules25194450
M3 - Article
C2 - 32998236
AN - SCOPUS:85092447796
SN - 1420-3049
VL - 25
JO - Molecules
JF - Molecules
IS - 19
M1 - 4450
ER -