Perturbation of platelet adhesion to endothelial cells by plasminogen activation in vitro

Hsiun Ing Chen, Yueh I. Wu, Yu Lun Hsieh, Guey Yueh Shi, Meei Jyh Jiang, Wen Chang Chang, Woei Jer Chuang, Wai Ming Kan, Ming Jer Tang, Chauying J. Jen

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1 引文 斯高帕斯(Scopus)


To investigate whether the endothelium-platelet interactions may be altered by plasminogen activation, cultured human umbilical vein endothelial cells (ECs) were treated with tissue-type plasminogen activator (t-PA) in the presence of plasminogen, and platelet adhesion to ECs was subsequently measured by using a tapered flow chamber. Our results demonstrated that platelets adhered more readily to t-PA treated EC monolayer than to the control monolayer at all shear stress levels tested. This phenomenon was treatment time-dependent and dose-dependent, and it could be blocked by adding plasmin inhibitors, such as ε-amino caproic acid and aprotinin. Adherent platelets on t-PA treated EC monolayer underwent more severe shape change than those on the control monolayer. While the extracellular matrix directly treated with t-PA attracted less platelets than the control matrix did, platelet adhesion to the matrix that was produced by t-PA-treated ECs was unaltered. These data suggest that t-PA treatment on ECs compromised antiplatelet-adhesion capability on their apical surface without altering the reactivity of their extracellular matrix towards platelets.

頁(從 - 到)934-938
期刊Thrombosis and Haemostasis
出版狀態已發佈 - 8月 1997

ASJC Scopus subject areas

  • 血液學


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