TY - JOUR
T1 - P3HT:Bebq2-based photovoltaic device enhances differentiation of hiPSC-derived retinal ganglion cells
AU - Hsu, Chih Chen
AU - Lin, Yi Ying
AU - Yang, Tien Chun
AU - Yarmishyn, Aliaksandr A.
AU - Lin, Tzu Wei
AU - Chang, Yuh Lih
AU - Hwang, De Kuang
AU - Wang, Chien Ying
AU - Liu, Yung Yang
AU - Lo, Wen Liang
AU - Peng, Chi Hsien
AU - Chen, Shih Jen
AU - Yang, Yi Ping
N1 - Funding Information:
Acknowledgments: We thank Aliaksandr Yarmishyn for critical English editing and review of the manuscript. This study was assisted in part by the Division of Experimental Surgery of the Department of Surgery and the Animal Center of Taipei Veterans General Hospital.
Funding Information:
This research was funded in part by the Novel Bioengineering and Technological Approaches to Solve Two Major Health Problems in Taiwan sponsored by the Taiwan Ministry of Science and Technology Academic Excellence Program (MOST 106-2633-B-009-001, MOST 107-2633-B-009-003). Ministry of Science and Technology (MOST 106-2319-B-001-003, MOST 107-2319-B-001-003, MOST 106-2119-M-010-001, MOST 107-2119-M-010-001, MOST 107-2320-B-010-023, MOST 106-3114-B-010-002. MOST 107-2321-B-010-007), Academia Sinica (106-0210-01-15-02, 107-0210-01-19-01), Taipei Veterans General Hospital (V106E-004-2, V106C-001, V107C-139 and V107E-002-2), TSGH (TSGH-C108-122), NDMC (MAB-108-049), AS Joint Research Program (VTA107-V1-5-1, VTA107-V1-5-2), TVGH-NTUH Joint Research Program (VN106-02 and VN107-16), VGHUST Joint Research Program (VGHUST107-G1-6-1), The Department of Health Cancer Center Research of Excellence (MOHW106-TDU-B-211-113001 and MOHW107-TDU-B-211-123001), National Health Research Institutes (NHRI-EX106-10621BI and NHRI-EX107-10621BI), Taiwan. This work was financially supported by the “Cancer Progression Research Center, National Yang-Ming University” from The Featured Areas Research Center Program within the framework of the Higher Education Sprout Project by the Ministry of Education (MOE) in Taiwan. This paper (work) is particularly supported by the Ministry of Education through the SPROUT Project-Center for Intelligent Drug Systems and Smart Bio-devices (IDS2B) of National Chiao-Tung University, Taiwan.
Funding Information:
Funding: This research was funded in part by the Novel Bioengineering and Technological Approaches to Solve Two Major Health Problems in Taiwan sponsored by the Taiwan Ministry of Science and Technology Academic Excellence Program (MOST 106-2633-B-009-001, MOST 107-2633-B-009-003). Ministry of Science and Technology (MOST 106-2319-B-001-003, MOST 107-2319-B-001-003, MOST 106-2119-M-010-001, MOST 107-2119-M-010-001, MOST 107-2320-B-010-023, MOST 106-3114-B-010-002. MOST 107-2321-B-010-007), Academia Sinica (106-0210-01-15-02, 107-0210-01-19-01), Taipei Veterans General Hospital (V106E-004-2, V106C-001, V107C-139 and V107E-002-2), TSGH (TSGH-C108-122), NDMC (MAB-108-049), AS Joint Research Program (VTA107-V1-5-1, VTA107-V1-5-2), TVGH-NTUH Joint Research Program (VN106-02 and VN107-16), VGHUST Joint Research Program (VGHUST107-G1-6-1), The Department of Health Cancer Center Research of Excellence (MOHW106-TDU-B-211-113001 and MOHW107-TDU-B-211-123001), National Health Research Institutes (NHRI-EX106-10621BI and NHRI-EX107-10621BI), Taiwan. This work was financially supported by the “Cancer Progression Research Center, National Yang-Ming University” from The Featured Areas Research Center Program within the framework of the Higher Education Sprout Project by the Ministry of Education (MOE) in Taiwan. This paper (work) is particularly supported by the Ministry of Education through the SPROUT Project-Center for Intelligent Drug Systems and Smart Bio-devices (IDS2B) of National Chiao-Tung University, Taiwan.
Publisher Copyright:
© 2019 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2019/6/1
Y1 - 2019/6/1
N2 - Electric field stimulation is known to affect various cellular processes, including cell fate specification and differentiation, particularly towards neuronal lineages. This makes it a promising therapeutic strategy to stimulate regeneration of neuronal tissues. Retinal ganglion cells (RGCs) is a type of neural cells of the retina responsible for transduction of visual signals fromthe retina to the brain cortex, and is often degenerated in various blindness-causing retinal diseases. The organic photovoltaic materials such as poly-3-hexylthiophene (P3HT) can generate electric current upon illumination with light of the visible spectrum, and possesses several advantageous properties, including light weight, flexibility and high biocompatibility, which makes them a highly promising tool for electric stimulation of cells in vitro and in vivo. In this study, we tested the ability to generate photocurrent by several formulations of blend (bulk heterojunction) of P3HT (which is electron donor material) with several electron acceptor materials, including Alq3 and bis(10-hydroxybenzo[h]quinolinato)beryllium (Bebq2). We found that the photovoltaic device based on bulk heterojunction of P3HT with Bebq2 could generate photocurrent when illuminated by both green laser and visible spectrum light. We tested the growth and differentiation capacity of human induced pluripotent stem cells (hiPSC)-derived RGCs when grown in interface with such photostimulated device, and found that they were significantly increased. The application of P3HT:Bebq2-formulation of photovoltaic device has a great potential for developments in retinal transplantation, nerve repair and tissue engineering approaches of treatment of retinal degeneration.
AB - Electric field stimulation is known to affect various cellular processes, including cell fate specification and differentiation, particularly towards neuronal lineages. This makes it a promising therapeutic strategy to stimulate regeneration of neuronal tissues. Retinal ganglion cells (RGCs) is a type of neural cells of the retina responsible for transduction of visual signals fromthe retina to the brain cortex, and is often degenerated in various blindness-causing retinal diseases. The organic photovoltaic materials such as poly-3-hexylthiophene (P3HT) can generate electric current upon illumination with light of the visible spectrum, and possesses several advantageous properties, including light weight, flexibility and high biocompatibility, which makes them a highly promising tool for electric stimulation of cells in vitro and in vivo. In this study, we tested the ability to generate photocurrent by several formulations of blend (bulk heterojunction) of P3HT (which is electron donor material) with several electron acceptor materials, including Alq3 and bis(10-hydroxybenzo[h]quinolinato)beryllium (Bebq2). We found that the photovoltaic device based on bulk heterojunction of P3HT with Bebq2 could generate photocurrent when illuminated by both green laser and visible spectrum light. We tested the growth and differentiation capacity of human induced pluripotent stem cells (hiPSC)-derived RGCs when grown in interface with such photostimulated device, and found that they were significantly increased. The application of P3HT:Bebq2-formulation of photovoltaic device has a great potential for developments in retinal transplantation, nerve repair and tissue engineering approaches of treatment of retinal degeneration.
KW - Induced pluripotent stem cells (iPSC)
KW - Photovoltaic
KW - Poly-3-hexylthiophene (P3HT)
KW - Retinal ganglia cells (RGC)
UR - http://www.scopus.com/inward/record.url?scp=85066922112&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85066922112&partnerID=8YFLogxK
U2 - 10.3390/ijms20112661
DO - 10.3390/ijms20112661
M3 - Article
C2 - 31151170
AN - SCOPUS:85066922112
SN - 1661-6596
VL - 20
JO - International journal of molecular sciences
JF - International journal of molecular sciences
IS - 11
M1 - 2661
ER -
