TY - JOUR
T1 - P-NMR analysis of the B to Z transition in double-stranded (dC-dG)3, and (dC-dG)4 in high salt solution
AU - Holak, Tadeusz A.
AU - Borer, Philip N.
AU - Levy, George C.
AU - van Boom, Jacques H.
AU - Wang, Andrew H.J.
N1 - Funding Information:
We thank Anil Kumar for discussion and assistance with the MOLDYN calculations, Dr. M. Petersheim for supplying unpublished data and Dr. L. S. Ka.i for a preprint. This work was supported by NIHGrant GM32691 (to PNB), NIHGrants GM29778, RR01317, and NSF Grant 81-05109 (to GCL), NIH Grant CA29753 (AH-JW), and American Cancer So ;iety Grant NP 3348 (to AH-JW and A. Rich).
PY - 1984/6/11
Y1 - 1984/6/11
N2 - In 4H NaCl solutions (dC-dG)n (n=3,4; ̃9mM) exist as a mixture ot B and Z forms. The low and high field components of two 31P NMR resonances originating from internal phosphodiester groups are assigned to the GpC and CpG linkages, respectively. Low temperatures stabilize the Z-forms, which completely disappear above 50°C (n=3) and 65°C (n=4). ΔH=-44 and -17 kJ/mol for B to Z transition in the hexamer and octamer duplexes, respectively. Temperature dependent changes (0-57°C range) in the spin-lattice relaxation times at 145.7 MHz are distinctly different for the 31p nuclei o± GpC and CpG groups. The relaxation data can be explained by assuming that the GpC phosphodiester groups undergo more local internal motion than do the CpG groups.
AB - In 4H NaCl solutions (dC-dG)n (n=3,4; ̃9mM) exist as a mixture ot B and Z forms. The low and high field components of two 31P NMR resonances originating from internal phosphodiester groups are assigned to the GpC and CpG linkages, respectively. Low temperatures stabilize the Z-forms, which completely disappear above 50°C (n=3) and 65°C (n=4). ΔH=-44 and -17 kJ/mol for B to Z transition in the hexamer and octamer duplexes, respectively. Temperature dependent changes (0-57°C range) in the spin-lattice relaxation times at 145.7 MHz are distinctly different for the 31p nuclei o± GpC and CpG groups. The relaxation data can be explained by assuming that the GpC phosphodiester groups undergo more local internal motion than do the CpG groups.
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U2 - 10.1093/nar/12.11.4625
DO - 10.1093/nar/12.11.4625
M3 - Article
C2 - 6547530
AN - SCOPUS:0021760444
SN - 0305-1048
VL - 12
SP - 4625
EP - 4635
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 11
ER -