OmpA is the critical component for escherichia coli invasion-induced astrocyte activation

Hsueh Hsia Wu, Yi Yuan Yang, Wen Shyang Hsieh, Chi Hsin Lee, Sy Jye C Leu, Mei Ru Chen

研究成果: 雜誌貢獻文章同行評審

19 引文 斯高帕斯(Scopus)


Escherichia coli is the major Gram-negative bacterial pathogen in neonatal meningitis. Outer membrane protein A (OmpA) is a conserved major protein in the E. coli outer membrane and is involved in several host-cell interactions. To characterize the role of OmpA in the invasion of astrocytes by E. coli, we investigated OmpA-positive and OmpA-negative E. coli strains. Outer membrane protein A E44, E105, and E109 strains adhered to and invaded C6 glioma cells 10- to 15-fold more efficiently than OmpA-negative strains. Actin rearrangement, protein tyrosine kinase, and phosphoinositide 3-kinase activation were required for OmpA-mediated invasion by E. coli. In vitro infection of C6 cells and intracerebral injection into mice of the E44 strain induced expression of the astrocyte differentiation marker glial fibrillary acidic protein and the inflammatory mediators cyclooxygenase 2 and nitric oxide synthase 2. After intracerebral infection with E44, all C57BL/6 mice died within 36hours, whereas 80% of mice injected with E44 premixed with recombinant OmpA protein survived. Astrocyte activation and neutrophil infiltration were reduced in brain tissue sections in the mice given OmpA. Taken together, these data suggest that OmpA-mediated invasion plays an important role in the early stage of E.coli-induced brain damage, and that it may have therapeutic use in E. coli meningitis.
頁(從 - 到)677-690
期刊Journal of Neuropathology and Experimental Neurology
出版狀態已發佈 - 6月 2009

ASJC Scopus subject areas

  • 病理學與法醫學
  • 神經病學(臨床)
  • 神經內科
  • 細胞與分子神經科學


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