摘要
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a potent immunomodulatory cytokine. Here we generated a novel codon-optimized murine GM-CSF gene as an adjuvant. The codon-optimized GM-CSF gene significantly increased protein expression levels in all cells tested. Although injection of the wild-type GM-CSF plasmids adjuvanted HIV-1 Gag DNA vaccine induced detectable immune responses, co-administration of plasmids encoding the codon-optimized GM-CSF sequence with the DNA vaccine resulted in a strong antibody and CTL responses and a protective immune response against infection with recombinant vaccinia virus expressing HIV-1 Gag. This novel codon-optimized GM-CSF gene offers a practical molecular strategy for potentiating immune responses to vaccines as well as other immunotherapeutic strategies.
原文 | 英語 |
---|---|
頁(從 - 到) | 253-263 |
頁數 | 11 |
期刊 | Vaccine |
卷 | 25 |
發行號 | 2 |
DOIs | |
出版狀態 | 已發佈 - 1月 4 2007 |
ASJC Scopus subject areas
- 分子醫學
- 免疫學與微生物學 (全部)
- 獸醫 (全部)
- 公共衛生、環境和職業健康
- 傳染性疾病