TY - JOUR
T1 - New insight into natural extracellular matrix
T2 - Genipin cross-linked adipose-derived stem cell extracellular matrix gel for tissue engineering
AU - Nyambat, Batzaya
AU - Manga, Yankuba B.
AU - Chen, Chih Hwa
AU - Gankhuyag, Uuganbayar
AU - Andi Pratomo, W. P.
AU - Satapathy, Mantosh Kumar
AU - Chuang, Er Yuan
N1 - Publisher Copyright:
© 2020 by the authors.
PY - 2020/7/9
Y1 - 2020/7/9
N2 - The cell-derived extracellular matrix (ECM) is associated with a lower risk of pathogen transfer, and it possesses an ideal niche with growth factors and complex fibrillar proteins for cell attachment and growth. However, the cell-derived ECM is found to have poor biomechanical properties, and processing of cell-derived ECM into gels is scarcely studied. The gel provides platforms for three-dimensional cell culture, as well as injectable biomaterials, which could be delivered via a minimally invasive procedure. Thus, in this study, an adipose-derived stem cell (ADSC)-derived ECM gel was developed and cross-linked by genipin to address the aforementioned issue. The genipin cross-linked ADSC ECM gel was fabricated via several steps, including rabbit ADSC culture, cell sheets, decellularization, freeze–thawing, enzymatic digestion, neutralization of pH, and cross-linking. The physicochemical characteristics and cytocompatibility of the gel were evaluated. The results demonstrated that the genipin cross-linking could significantly enhance the mechanical properties of the ADSC ECM gel. Furthermore, the ADSC ECM was found to contain collagen, fibronectin, biglycan, and transforming growth factor (TGF)-β1, which could substantially maintain ADSC, skin, and ligament fibroblast cell proliferation. This cell-derived natural material could be suitable for future regenerative medicine and tissue engineering application.
AB - The cell-derived extracellular matrix (ECM) is associated with a lower risk of pathogen transfer, and it possesses an ideal niche with growth factors and complex fibrillar proteins for cell attachment and growth. However, the cell-derived ECM is found to have poor biomechanical properties, and processing of cell-derived ECM into gels is scarcely studied. The gel provides platforms for three-dimensional cell culture, as well as injectable biomaterials, which could be delivered via a minimally invasive procedure. Thus, in this study, an adipose-derived stem cell (ADSC)-derived ECM gel was developed and cross-linked by genipin to address the aforementioned issue. The genipin cross-linked ADSC ECM gel was fabricated via several steps, including rabbit ADSC culture, cell sheets, decellularization, freeze–thawing, enzymatic digestion, neutralization of pH, and cross-linking. The physicochemical characteristics and cytocompatibility of the gel were evaluated. The results demonstrated that the genipin cross-linking could significantly enhance the mechanical properties of the ADSC ECM gel. Furthermore, the ADSC ECM was found to contain collagen, fibronectin, biglycan, and transforming growth factor (TGF)-β1, which could substantially maintain ADSC, skin, and ligament fibroblast cell proliferation. This cell-derived natural material could be suitable for future regenerative medicine and tissue engineering application.
KW - Cell-derived
KW - Cross-linked
KW - Extracellular matrix gel
KW - Genipin
KW - Tissue engineering
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U2 - 10.3390/ijms21144864
DO - 10.3390/ijms21144864
M3 - Article
C2 - 32660134
AN - SCOPUS:85087759574
SN - 1661-6596
VL - 21
SP - 1
EP - 18
JO - International journal of molecular sciences
JF - International journal of molecular sciences
IS - 14
M1 - 4864
ER -