TY - JOUR
T1 - Nanocatalyst coupled with a latent-ratiometric electrochemical switch for label-free zero-tolerance rapid detection of live Salmonella in whole blood samples
AU - Kumaragurubaran, Namasivayam
AU - Arul, P.
AU - Huang, Sheng Tung
AU - Huang, Chih Hung
AU - Fang, Shiuh Bin
AU - Lin, Ying Hsiu
N1 - Funding Information:
The authors are grateful for financial support from the Ministry of Science and Technology, Taiwan (MOST-107-2113-M-027-006 and MOST-108-2113-M-027-001). P. Arul would like to thank the National Taipei University of Technology for the position of Research Assistant Professor.
Funding Information:
The authors are grateful for financial support from the Ministry of Science and Technology, Taiwan ( MOST-107-2113-M-027-006 and MOST-108-2113-M-027-001 ). P. Arul would like to thank the National Taipei University of Technology for the position of Research Assistant Professor.
Publisher Copyright:
© 2023 Elsevier B.V.
PY - 2023/4/15
Y1 - 2023/4/15
N2 - Developing a simple and rapid analytical tool for detecting live Salmonella is urgently needed for clinical diagnosis and food surveillance. In our previous studies, an activity-based latent ratiometric electrochemical switch was successfully deployed to monitor Salmonella but it could not achieve “zero-tolerance” detection; thus, improving the detection sensitivity from that of our previous approach is needed in order to obtain a practical tool for detecting Salmonella. In this study, a latent ratiometric electrochemical switch Sal-CAF coupled with graphene quantum dots-gold nanoparticles (GQDs-AuNPs) modified electrode was developed to meet the needs of selective detection of live Salmonella. Stable and uniformly sized GQDs-AuNPs (14.25 ± 0.2 nm) were achieved by using a free of reducing agent. The Sal-CAF coupled with GQDs-AuNPs/GCE showed an enhanced current response with an extended linear Salmonella detection concentration range of 1.0 × 103 to 1.0 × 1010 CFU/mL and a limit of detection of 35.62 × 101 CFU/mL within 4 h. Moreover, the detection of 100 CFU/mL of the bacteria could be achieved through a dynamic monitoring method in under 12 h without sample pretreatment or enrichment. Additionally, combining GQDs-AuNPs/GCE with the Sal-CAF platform achieved excellent selectivity, serotypes of pathogenic bacteria, detection of dry-emphasized Salmonella without carrying out a pre-enrichment process, the ability to distinguish live from dead cells, and the ability to work in turbid whole blood media. The present approach constitutes a new achievement for the concept of “zero-tolerance,” as it can be used to detect bacterial pathogens ahead of infection control.
AB - Developing a simple and rapid analytical tool for detecting live Salmonella is urgently needed for clinical diagnosis and food surveillance. In our previous studies, an activity-based latent ratiometric electrochemical switch was successfully deployed to monitor Salmonella but it could not achieve “zero-tolerance” detection; thus, improving the detection sensitivity from that of our previous approach is needed in order to obtain a practical tool for detecting Salmonella. In this study, a latent ratiometric electrochemical switch Sal-CAF coupled with graphene quantum dots-gold nanoparticles (GQDs-AuNPs) modified electrode was developed to meet the needs of selective detection of live Salmonella. Stable and uniformly sized GQDs-AuNPs (14.25 ± 0.2 nm) were achieved by using a free of reducing agent. The Sal-CAF coupled with GQDs-AuNPs/GCE showed an enhanced current response with an extended linear Salmonella detection concentration range of 1.0 × 103 to 1.0 × 1010 CFU/mL and a limit of detection of 35.62 × 101 CFU/mL within 4 h. Moreover, the detection of 100 CFU/mL of the bacteria could be achieved through a dynamic monitoring method in under 12 h without sample pretreatment or enrichment. Additionally, combining GQDs-AuNPs/GCE with the Sal-CAF platform achieved excellent selectivity, serotypes of pathogenic bacteria, detection of dry-emphasized Salmonella without carrying out a pre-enrichment process, the ability to distinguish live from dead cells, and the ability to work in turbid whole blood media. The present approach constitutes a new achievement for the concept of “zero-tolerance,” as it can be used to detect bacterial pathogens ahead of infection control.
KW - Bacterial-pathogens
KW - Dry-emphasized
KW - Electrochemical switch
KW - Salmonella
KW - Zero-tolerance
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U2 - 10.1016/j.snb.2023.133428
DO - 10.1016/j.snb.2023.133428
M3 - Article
AN - SCOPUS:85147225810
SN - 0925-4005
VL - 381
JO - Sensors and Actuators B: Chemical
JF - Sensors and Actuators B: Chemical
M1 - 133428
ER -