NanoBioAnalytical characterization of extracellular vesicles in 75-nm nanofiltered human plasma for transfusion: A tool to improve transfusion safety

Sameh Obeid; Pei Shan Sung; Benoit Le Roy; Ming Li Chou; Shie Liang Hsieh; Celine Elie-Caille; Thierry Burnouf; Wilfrid Boireau

doi:10.1016/j.nano.2019.02.026

NanoBioAnalytical characterization of extracellular vesicles in 75-nm nanofiltered human plasma for transfusion: A tool to improve transfusion safety

Sameh Obeid, Pei Shan Sung, Benoit Le Roy, Ming Li Chou, Shie Liang Hsieh, Celine Elie-Caille, Thierry Burnouf, Wilfrid Boireau

研究成果: 雜誌貢獻 › 文章 › 同行評審

17 引文斯高帕斯（Scopus）

摘要

Plasma transfusion induces some transfusion related acute lung injury (TRALI) mediated through neutrophil extracellular traps (NETs). We investigated whether extracellular vesicles (EVs) present in plasma or obtained from resting (N-PEVs) or thrombin activated platelets (T-PEVs) can trigger NETs, and whether 75 nm-nanofiltration, to partially remove EVs, prohibits NETs formation. EVs size and concentration were determined by conventional biophysical approaches and by an original NanoBioAnalytical (NBA) platform based on EV immunocapture biochip, combining Surface Plasmon Resonance Imaging (SPRi) and Atomic Force Microscopy (AFM) exploration. EVs effective diameter was in the 25-1000 nm range, with a majority (≈ 90%) ≤ 100 nm. Both T-PEVs in buffer (but not N-PEVs) and non-nanofiltered plasma containing T-PEVs triggered NETs formation. Nanofiltration depleted large EVs (> 70 nm) and decreased NETs formation. The NBA platform was found to be a suitable tool to investigate the safety of plasma for transfusion.

原文	英語
文章編號	101977
頁（從 - 到）	101977
期刊	Nanomedicine: Nanotechnology, Biology, and Medicine
卷	20
DOIs	https://doi.org/10.1016/j.nano.2019.02.026
出版狀態	已發佈 - 8月 1 2019

ASJC Scopus subject areas

生物工程
醫藥（雜項）
分子醫學
生物醫學工程
一般材料科學
藥學科學

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10.1016/j.nano.2019.02.026

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Obeid, S., Sung, P. S., Le Roy, B., Chou, M. L., Hsieh, S. L., Elie-Caille, C., Burnouf, T., & Boireau, W. (2019). NanoBioAnalytical characterization of extracellular vesicles in 75-nm nanofiltered human plasma for transfusion: A tool to improve transfusion safety. Nanomedicine: Nanotechnology, Biology, and Medicine, 20, 101977. 文章 101977. https://doi.org/10.1016/j.nano.2019.02.026

Obeid, S, Sung, PS, Le Roy, B, Chou, ML, Hsieh, SL, Elie-Caille, C, Burnouf, T & Boireau, W 2019, 'NanoBioAnalytical characterization of extracellular vesicles in 75-nm nanofiltered human plasma for transfusion: A tool to improve transfusion safety', Nanomedicine: Nanotechnology, Biology, and Medicine, 卷 20, 101977, 頁 101977. https://doi.org/10.1016/j.nano.2019.02.026

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abstract = "Plasma transfusion induces some transfusion related acute lung injury (TRALI) mediated through neutrophil extracellular traps (NETs). We investigated whether extracellular vesicles (EVs) present in plasma or obtained from resting (N-PEVs) or thrombin activated platelets (T-PEVs) can trigger NETs, and whether 75 nm-nanofiltration, to partially remove EVs, prohibits NETs formation. EVs size and concentration were determined by conventional biophysical approaches and by an original NanoBioAnalytical (NBA) platform based on EV immunocapture biochip, combining Surface Plasmon Resonance Imaging (SPRi) and Atomic Force Microscopy (AFM) exploration. EVs effective diameter was in the 25-1000 nm range, with a majority (≈ 90%) ≤ 100 nm. Both T-PEVs in buffer (but not N-PEVs) and non-nanofiltered plasma containing T-PEVs triggered NETs formation. Nanofiltration depleted large EVs (> 70 nm) and decreased NETs formation. The NBA platform was found to be a suitable tool to investigate the safety of plasma for transfusion.",

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NanoBioAnalytical characterization of extracellular vesicles in 75-nm nanofiltered human plasma for transfusion: A tool to improve transfusion safety

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