@article{789b5ed4c7164ed3856a681071e23e66,
title = "Muscle atrophy-related myotube-derived exosomal microRNA in neuronal dysfunction: Targeting both coding and long noncoding RNAs",
abstract = "In mammals, microRNAs can be actively secreted from cells to blood. miR-29b-3p has been shown to play a pivotal role in muscle atrophy, but its role in intercellular communication is largely unknown. Here, we showed that miR-29b-3p was upregulated in normal and premature aging mouse muscle and plasma. miR-29b-3p was also upregulated in the blood of aging individuals, and circulating levels of miR-29b-3p were negatively correlated with relative appendicular skeletal muscle. Consistently, miR-29b-3p was observed in exosomes isolated from long-term differentiated atrophic C2C12 cells. When C2C12-derived miR-29b-3p-containing exosomes were uptaken by neuronal SH-SY5Y cells, increased miR-29b-3p levels in recipient cells were observed. Moreover, miR-29b-3p overexpression led to downregulation of neuronal-related genes and inhibition of neuronal differentiation. Interestingly, we identified HIF1α-AS2 as a novel c-FOS targeting lncRNA that is induced by miR-29b-3p through down-modulation of c-FOS and is required for miR-29b-3p-mediated neuronal differentiation inhibition. Our results suggest that atrophy-associated circulating miR-29b-3p may mediate distal communication between muscle cells and neurons.",
keywords = "aging, HIF-1α-AS2, lncRNAs, miR-29b-3p, muscle atrophy",
author = "Yang, {Chia Pei} and Yang, {Wan Shan} and Wong, {Yu Hui} and Wang, {Kai Hsuan} and Teng, {Yuan Chi} and Chang, {Ming Hsuan} and Liao, {Ko Hsun} and Nian, {Fang Shin} and Chao, {Chuan Chuan} and Tsai, {Jin Wu} and Hwang, {Wei Lun} and Lin, {Ming Wei} and Tzeng, {Tsai Yu} and Wang, {Pei Ning} and Mel Campbell and Chen, {Liang Kung} and Tsai, {Ting Fen} and Chang, {Pei Ching} and Kung, {Hsing Jien}",
note = "Funding Information: This paper is dedicated memorializing Dr. Hsei-Wei Wang, Apr 1969 ~ Sep 2015, who initiated this project in 2014. The work was supported by grants (MOST 105-2633-B-400-001-003 to HJK, PCC, and TFT; MOST 105-2320-B-038-071-MY3, MOST 106-2314-B-038-093 and 107-2320-B-038-055-MY3 to HJK; MOST 105-2320-B-010-007-MY3 to PCC; MOST 107-3011-B-010-001 to TFT, and MOST-108-2321-B-010-013-MY2 to PNW). This work was also financially supported by the “Cancer Progression Research Center, National Yang-Ming University” and “Cancer Center, Taipei Medical University” from The Featured Areas Research Center Program within the framework of the Higher Education Sprout Project by the Ministry of Education (MOE) in Taiwan to PCC and HJK, respectively. The authors acknowledge the “High-throughput Genome and Big Data Analysis Core Facility of National Core Facility Program for Biotechnology, Taiwan (MOST 105-2319-B-010-001)” for high-throughput sequencing and the “Cancer Progression Research Center-Genome Editing Core Facility of Featured Areas Research Center Program within the framework of the Higher Education Sprout Project by MOE” for CISD2 CRISPR KO C2C12 cell line. We thank Yi-Lin Chung and Ting-Kuan Chu for their help with the histological analyses of Figure a and Figure S5, respectively. We acknowledge the strong support of the NTU-AS joint laboratory from Dr. Yen Yun, TMU, AS IBC, and NTU IBS. Funding Information: This paper is dedicated memorializing Dr. Hsei‐Wei Wang, Apr 1969 ~ Sep 2015, who initiated this project in 2014. The work was supported by grants (MOST 105‐2633‐B‐400‐001‐003 to HJK, PCC, and TFT; MOST 105‐2320‐B‐038‐071‐MY3, MOST 106‐2314‐B‐038‐093 and 107‐2320‐B‐038‐055‐MY3 to HJK; MOST 105‐2320‐B‐010‐007‐MY3 to PCC; MOST 107‐3011‐B‐010‐001 to TFT, and MOST‐108‐2321‐B‐010‐013‐MY2 to PNW). This work was also financially supported by the “Cancer Progression Research Center, National Yang‐Ming University” and “Cancer Center, Taipei Medical University” from The Featured Areas Research Center Program within the framework of the Higher Education Sprout Project by the Ministry of Education (MOE) in Taiwan to PCC and HJK, respectively. The authors acknowledge the “High‐throughput Genome and Big Data Analysis Core Facility of National Core Facility Program for Biotechnology, Taiwan (MOST 105‐2319‐B‐010‐001)” for high‐throughput sequencing and the “Cancer Progression Research Center‐Genome Editing Core Facility of Featured Areas Research Center Program within the framework of the Higher Education Sprout Project by MOE” for CISD2 CRISPR KO C2C12 cell line. We thank Yi‐Lin Chung and Ting‐Kuan Chu for their help with the histological analyses of Figure a and Figure S5 , respectively. We acknowledge the strong support of the NTU‐AS joint laboratory from Dr. Yen Yun, TMU, AS IBC, and NTU IBS. Publisher Copyright: {\textcopyright} 2020 The Authors. Aging Cell published by the Anatomical Society and John Wiley & Sons Ltd.",
year = "2020",
month = may,
day = "1",
doi = "10.1111/acel.13107",
language = "English",
volume = "19",
journal = "Aging Cell",
issn = "1474-9718",
publisher = "Wiley-Blackwell",
number = "5",
}