TY - JOUR
T1 - Multidisciplinary approach in study of the zoonotic Anisakis larval infection in the blue mackerel (Scomber australasicus) and the largehead hairtail (Trichiurus lepturus) in Northern Taiwan
AU - Sonko, Pasaikou
AU - Chih-Cheng Chen, Solomon
AU - Chou, Chia Mei
AU - Huang, Ying Chieh
AU - Hsu, Shao Lun
AU - Barčák, Daniel
AU - Oros, Mikuláš
AU - Fan, Chia Kwung
N1 - Funding Information:
The following individuals provided valuable assistance for the fish's dissection and parasite collection: Ka-Chon Ng, Yu-Hsuan Chen, Hsun-Yi Wang, Cheng- Yen Shih (Taipei Medical University); Jen-Ping Hsiao, Chi-Yang Lo (National Taiwan Normal University). This project was funded by Ditmanson Medical Foundation Chia-Yi Christian Hospital Research Program ( R104-017 ) and a joint project by Slovak Academy of Sciences and Taiwan Ministry of Science and Technology (SAS- MOST JRP 2016/7 , MOST 106-2923-B-038 -001-MY3 ).
Funding Information:
The following individuals provided valuable assistance for the fish's dissection and parasite collection: Ka-Chon Ng, Yu-Hsuan Chen, Hsun-Yi Wang, Cheng- Yen Shih (Taipei Medical University); Jen-Ping Hsiao, Chi-Yang Lo (National Taiwan Normal University). This project was funded by Ditmanson Medical Foundation Chia-Yi Christian Hospital Research Program (R104-017) and a joint project by Slovak Academy of Sciences and Taiwan Ministry of Science and Technology (SAS-MOST JRP 2016/7, MOST 106-2923-B-038 -001-MY3).
Publisher Copyright:
© 2019
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Background: Anisakid larvae are the food-borne pathogen highly prevalent among numerous marine fishes. Accidental consumption of infected raw or poorly cooked fish fillets may cause anisakiasis. Methods: This study used the multidisciplinary approach to investigate the occurrence of Anisakis nematodes in commonly consumed fish species, Scomber australasicus and Trichiurus lepturus purchased in Taipei Xinyi traditional fish market. Results: All the Anisakis larvae collected herein were identified morphologically as Anisakis type I or Anisakis type II. The prevalence and the mean intensity of Anisakis larvae collected from S. australasicus was 80.77%, 26.8 (10–32) and 100%, 49.0 (27–70) for T. lepturus. Using molecular analysis, 83.33% (180/216) were identified as Anisakis pegreffii, 6.05% (13/216) as Ascaris typica, 1.85% (4/216) as Ascaris physeteris and 8.80% (19/216) as hybrid genotype (A. pegreffii + Anisakis simplex) in S. australasicus. In T. lepturus, 86.31% (290/336) were identified as A. pegreffii, 2.38% (8/336) as A. typica, and 11.31% (38/336) as hybrid genotype (A. pegreffii + A. simplex [s.s]). The molecular phylogenetic analysis shows two cluster clades, one group includes A. pegreffii complex and the other include Ascaris paggiae, Ascaris brevispiculata, and A. physeteris. Conclusion: Thus, A. pegreffii is the most abundant species and may be the potential causes of human infection.
AB - Background: Anisakid larvae are the food-borne pathogen highly prevalent among numerous marine fishes. Accidental consumption of infected raw or poorly cooked fish fillets may cause anisakiasis. Methods: This study used the multidisciplinary approach to investigate the occurrence of Anisakis nematodes in commonly consumed fish species, Scomber australasicus and Trichiurus lepturus purchased in Taipei Xinyi traditional fish market. Results: All the Anisakis larvae collected herein were identified morphologically as Anisakis type I or Anisakis type II. The prevalence and the mean intensity of Anisakis larvae collected from S. australasicus was 80.77%, 26.8 (10–32) and 100%, 49.0 (27–70) for T. lepturus. Using molecular analysis, 83.33% (180/216) were identified as Anisakis pegreffii, 6.05% (13/216) as Ascaris typica, 1.85% (4/216) as Ascaris physeteris and 8.80% (19/216) as hybrid genotype (A. pegreffii + Anisakis simplex) in S. australasicus. In T. lepturus, 86.31% (290/336) were identified as A. pegreffii, 2.38% (8/336) as A. typica, and 11.31% (38/336) as hybrid genotype (A. pegreffii + A. simplex [s.s]). The molecular phylogenetic analysis shows two cluster clades, one group includes A. pegreffii complex and the other include Ascaris paggiae, Ascaris brevispiculata, and A. physeteris. Conclusion: Thus, A. pegreffii is the most abundant species and may be the potential causes of human infection.
KW - Anisakiasis
KW - Fish-borne parasite
KW - Molecular phylogeny
KW - Scomber australasicus
KW - Trichiurus lepturus
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U2 - 10.1016/j.jmii.2019.04.012
DO - 10.1016/j.jmii.2019.04.012
M3 - Article
AN - SCOPUS:85066832879
SN - 1684-1182
VL - 53
SP - 1021
EP - 1029
JO - Journal of Microbiology, Immunology and Infection
JF - Journal of Microbiology, Immunology and Infection
IS - 6
ER -