摘要
AU-rich element binding factor 1 (AUF1) has a role in the replication cycles of different viruses. Here we demonstrate that AUF1 binds the internal ribosome entry site (IRES) of enterovirus 71 (EV71) and negatively regulates IRES-dependent translation. During EV71 infection, AUF1 accumulates in the cytoplasm where viral replication occurs, whereas AUF1 localizes predominantly in the nucleus in mock-infected cells. AUF1 knockdown in infected cells increases IRES activity and synthesis of viral proteins. Taken together, the results suggest that AUF1 interacts with the EV71 IRES to negatively regulate viral translation and replication.
原文 | 英語 |
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文章編號 | e103827 |
期刊 | PLoS ONE |
卷 | 9 |
發行號 | 7 |
DOIs | |
出版狀態 | 已發佈 - 7月 31 2014 |
ASJC Scopus subject areas
- 多學科
- 一般生物化學,遺傳學和分子生物學
- 一般農業與生物科學