TY - JOUR
T1 - Molecular structure of nicked DNA
T2 - A substrate for DNA repair enzymes
AU - Aymami, J.
AU - Coll, M.
AU - Van Der Marel, G. A.
AU - Van Boom, J. H.
AU - Wang, A. H.J.
AU - Rich, A.
PY - 1990
Y1 - 1990
N2 - The molecular structure of a nicked dodecamer DNA double helix, made of a ternary system containing d(CGCGAAAACGCG) + d(CGCGTT) + d(TTCGCG) oligonucleotides, has been determined by x-ray diffraction analysis at 3 Å resolution. The molecule adopts a B-DNA conformation, not unlike those found in intact dodecamer DNA molecules crystallized in a somewhat different crystal lattice, despite a gap due to the absence of a phosphate group in the molecule. The helix has a distinct narrow minor groove near the center of the molecule at the AAAA region. This suggests that the internal stabilizing forces due to base stacking and hydrogen-bonding interactions are sufficient to overcome the loss of connectivity associated with the disruption of the covalent backbone of DNA.
AB - The molecular structure of a nicked dodecamer DNA double helix, made of a ternary system containing d(CGCGAAAACGCG) + d(CGCGTT) + d(TTCGCG) oligonucleotides, has been determined by x-ray diffraction analysis at 3 Å resolution. The molecule adopts a B-DNA conformation, not unlike those found in intact dodecamer DNA molecules crystallized in a somewhat different crystal lattice, despite a gap due to the absence of a phosphate group in the molecule. The helix has a distinct narrow minor groove near the center of the molecule at the AAAA region. This suggests that the internal stabilizing forces due to base stacking and hydrogen-bonding interactions are sufficient to overcome the loss of connectivity associated with the disruption of the covalent backbone of DNA.
KW - DNA conformation
KW - x-ray diffraction
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U2 - 10.1073/pnas.87.7.2526
DO - 10.1073/pnas.87.7.2526
M3 - Article
C2 - 2320572
AN - SCOPUS:0025276075
SN - 0027-8424
VL - 87
SP - 2526
EP - 2530
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 7
ER -