TY - JOUR
T1 - Molecular structure of (m5 dC-dG)3
T2 - The role of the methyl group on 5-methyl cytosine in stabilizing Z-DNA
AU - Fujii, Satoshi
AU - Wang, Andrew H.J.
AU - van der Marel, Gijs
AU - van Boom, Jacques H.
AU - Rich, Alexander
N1 - Funding Information:
ACKNOWLEDGMENTS This research was supported by grants from the National Institutes of Health, the American Cancer Society, andd the National Aeronautics and Space Administration and Netherlands Organization for the Advancement of Pure Research (ZYO).
PY - 1982/12/11
Y1 - 1982/12/11
N2 - The hexamer (m5dC-dG)3 has been synthesized and its three-dimensional structure determined by a single crystal X-ray diffraction analysis. The structure has been refined to a final R valne of 15.6% at 1.3 Å resolution. The molecule forms a left-handed Z-DNA helix which is similar to the unmethylated Z-DNA structure. The presence of the methyl group has resulted in slight changes in the twist angle between snccessive base pairs and modification of some of the interatomic contacts. Methylation of cytosine in the C5 position is associated with a relative destabilization of the B-DNA structure and a stabilization through hydrophobic bonding of the Z-DNA structure.
AB - The hexamer (m5dC-dG)3 has been synthesized and its three-dimensional structure determined by a single crystal X-ray diffraction analysis. The structure has been refined to a final R valne of 15.6% at 1.3 Å resolution. The molecule forms a left-handed Z-DNA helix which is similar to the unmethylated Z-DNA structure. The presence of the methyl group has resulted in slight changes in the twist angle between snccessive base pairs and modification of some of the interatomic contacts. Methylation of cytosine in the C5 position is associated with a relative destabilization of the B-DNA structure and a stabilization through hydrophobic bonding of the Z-DNA structure.
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U2 - 10.1093/nar/10.23.7879
DO - 10.1093/nar/10.23.7879
M3 - Article
C2 - 7155900
AN - SCOPUS:0020480809
SN - 0305-1048
VL - 10
SP - 7879
EP - 7892
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 23
ER -