Molecular structure of an A‐DNA decamer d(ACCGGCCGGT)

Christin A. FREDERICK, Gary J. QUIGLEY, Mai‐Kun ‐K TENG, Miquel COLL, Gijs A. van der MAREL, Jacques H. van BOOM, Alexander RICH, Andrew H.‐J WANG

研究成果: 雜誌貢獻文章同行評審

60 引文 斯高帕斯(Scopus)


The molecular structure of the DNA decamer d(ACCGGCCGGT) has been solved and refined by single‐crystal X‐ray‐diffraction analysis at 0.20 nm to a final R‐factor of 18.0°. The decamer crystallizes as an A‐DNA double helical fragment with unit‐cell dimensions of a=b= 3.923 nm and c= 7.80 nm in the space group P6122. The overall conformation of this A‐DNA decamer is very similar to that of the fiber model for A‐DNA which has a large average base‐pair tilt and hence a wide and shallow minor groove. This structure is in contrast to that of several A‐DNA octamers in which the molecules all have low base‐pair‐tilt angles (8–12°) resulting in an appearance intermediate between B‐DNA and A‐DNA. The average helical parameters of this decamer are typical of A‐DNA with 10.9 base pairs/turn of helix, an average helical twist angle of 33.1°, and a base‐pair‐tilt angle of 18.2°. However, the CpG step in this molecule has a low local‐twist angle of 24.5°, similar to that seen in other A‐DNA oligomers, and therefore appears to be an intrinsic stacking pattern for this step. The molecules pack in the crystal using a recurring binding motif, namely, the terminal base pair of one helix abuts the surface of the shallow minor groove of another helix. In addition, the GC base pairs have large propeller‐twist angles, unlike those found most other A‐DNA structures.

頁(從 - 到)295-307
期刊European Journal of Biochemistry
出版狀態已發佈 - 5月 1989

ASJC Scopus subject areas

  • 生物化學


深入研究「Molecular structure of an A‐DNA decamer d(ACCGGCCGGT)」主題。共同形成了獨特的指紋。