Modulation of Monocyte-Derived Dendritic Cell Differentiation is Associated With Ischemic Acute Renal Failure

Chih Jen Wu, Joen Rong Sheu, Han Hsiang Chen, Hui Fen Liao, Yuh Cheng Yang, Stone Yang, Yu Jen Chen

研究成果: 雜誌貢獻文章同行評審

13 引文 斯高帕斯(Scopus)


Background: Dendritic cells (DCs) play a central role in both stimulating and suppressing immune responses and are impacted by surgical injury, exercise, and other physiological stressors. This study aims to determine whether renal ischemia/reperfusion (I/R) injury alters the differentiation, maturation, and activation of DCs from peripheral blood monocytes (PBMo). Materials and methods: Sprague-Dawley (SD) rats were subjected to I/R injury or sham-operated. Creatinine clearance (CCr) was monitored daily during the 14 days of reperfusion that followed the ischemic insult. At 2 and 14 days of reperfusion, the following properties of PBMo derived-DCs were assessed: the amount of generated DCs, surface markers [CD11c, CD80, CD86, and MHC-II (IA)], and functional status including magnitude of mixed lymphocyte reaction (MLR), production of IL-12 p70 by DCs, and production of IFN-γ and IL-4 by DC-stimulated T cells. Results: CCr was greatly reduced in the injured rats 0 to 4 days after ischemia. Two days after I/R injury to kidney, the numbers of DCs differentiated from PBMo, IL-12 production by DCs, expression of MHC-II (IA), and IFN-γ production by DC-stimulated T cells were significantly increased in the I/R injured group (compared to the sham-operated group). After 14 days of reperfusion, there was no between-group differences in the numbers of DCs derived from PBMo, MLR, expression of CD80, CD86, and MHC-II (IA), and production of IL-12, IFN-γ, and IL-4. Conclusions: The increases seen at 2 days of reperfusion may reflect a preparatory step in the renal I/R injury pathway. The relationship between up-regulation of DC differentiation and ischemic acute renal failure (ARF) remains to be elucidated.

頁(從 - 到)104-111
期刊Journal of Surgical Research
出版狀態已發佈 - 5月 2006

ASJC Scopus subject areas

  • 手術


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