Modulation of 6-Thioguanine Activity by Guanine in Human Promyelocytic Leukemia HL-60 Cells1

Hui Wen Cheng, R. Douglas Armstrong, Wolfgang Sadae

研究成果: 雜誌貢獻文章同行評審

5 引文 斯高帕斯(Scopus)


The effects of guanine coadministration on the metabolism and biological activity of 6-thioguanine (6-TG) were studied in human promyelocytic leukemia cells (HL-60). Cell growth, cytotoxicity (cloning assay), and cell differentiation were measured, along with nucleotide metabolism. Guanine was efficiently salvaged by HL-60 cells; at 200 μM,guanine suppressed the formation of 6-TG mononucleotides and abolished 6-TG incorporation into nucleic acids. Similarly, guanine antagonized 6-TG cytotoxicity in a dose dependent fashion. Furthermore, guanine (200 μM)fully suppressed the 6-TG (10 μm)induced HL-60 cell differentiation, which suggests that cell differentiation at pharmacological 6-TG concentrations is dependent on the anabolism of the drug to active nucleotides. 6-TG given alone reduced GTP levels and DNA synthesis rates in HL-60 cells, while a major intracellular 6-TG metabolite, 6-thioguanosine 5’-monophosphate, accumulated to high levels (~100 μm).It is suggested that accumulation of 6-thioguanosine 5’ -monophosphate and a resultant partial block of the de novo biosynthesis of guanine nucleotides is responsible for 6-TG induced cell differentiation in HL-60 cells.

頁(從 - 到)3648-3651
期刊Cancer Research
出版狀態已發佈 - 1988

ASJC Scopus subject areas

  • 腫瘤科
  • 癌症研究


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