摘要
The interrelationship between microglia and astrocytes in cerebral ischemia was determined in vitro by adding in vitro ischemia-induced supernatant from microglia into astrocytes under the same conditions (glucose-, oxygen- and serum-free). The involvement of glial cell line-derived neurotrophic factor (GDNF) was further investigated by immunoblocking assay and Western blot analysis. Results showed that microglia-derived supernatant protected against in vitro ischemia-induced damage of astrocytes and this protection was pre-blocked by anti-GDNF but not normal rabbit serum. In addition, in vitro ischemia appeared to induce the expression of GDNF in microglia. These results indicate that microglia-derived protection on astrocytes during in vitro ischemia is GDNF-dependent.
原文 | 英語 |
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頁(從 - 到) | 111-114 |
頁數 | 4 |
期刊 | Neuroscience Letters |
卷 | 356 |
發行號 | 2 |
DOIs | |
出版狀態 | 已發佈 - 2月 12 2004 |
ASJC Scopus subject areas
- 神經科學 (全部)