Masticatory hypofunction effects induced by BTXA injection of hippocampal neurons in developing rats

Chi Yang Tsai, Hsin Ping Lee, Hung Ming Chang, Fang Chun Wu

研究成果: 雜誌貢獻文章同行評審

3 引文 斯高帕斯(Scopus)


Background and objective: In clinical practice, malocclusion is often encountered during the period of growth and development of individuals. In addition to nutritional imbalance, some studies have found that mastication affects learning and memory ability. Tooth loss and masticatory hypofunction have been suggested as risk factors of Alzheimer disease. However, relatively little research has been done in developing animals. The present study evaluated the relationship between masticatory hypofunction and neuropathological changes of the hippocampus in developing rats. Design: Four-week-old Wistar rats were randomly divided into saline-injected and botulinum toxin type A (BTXA)-injected groups. After an experiment period of 4 weeks, the rats were sacrificed for evaluation of neuropathological changes in the hippocampus through Nissl staining and phosphorylated cyclic AMP (cAMP) response element binding protein (CREB) immunohistochemistry. Results: Nissl staining revealed a significant reduction in the density of neurons in the BTXA-injected rats. The BTXA-injected rats exhibited a decreased level of CREB phosphorylation. The degree of p-CREB immunoreactivity differed significantly between the two groups. Conclusion: The BTXA-injected rats exhibited a reduction in neuron density and phosphorylated CREB, indicating that mastication might influence the learning and memory ability during the growth period. Therefore, it is strongly suggested that malocclusion be corrected as soon as possible during growth and development.
頁(從 - 到)122-129
期刊Archives of Oral Biology
出版狀態已發佈 - 12月 1 2018

ASJC Scopus subject areas

  • 耳鼻咽喉科
  • 牙科 (全部)
  • 細胞生物學


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