Magnolol encapsulated by liposome in inhibiting smooth muscle cell proliferation

Magnolol, a pure compound extracted from Magnolia officinalis, encapsulated by liposome was investigated for inhibiting vascular smooth muscle cell (VSMC) proliferation leading to restenosis by Percutaneous Transluminal Coronary Angioplasty (PTCA). 1,2-Diacyl-Sn-glycero-3-phosphocholine (EPC) and 1,2-dipalmitoyl-Sn-glycero-3-phosphocholine (DPPC) liposomes were utilized to encapsulate the magnolol in this study. The inhibitory efficiency of the liposome encapsulated magnolol on cell viability was higher than the pure magnolol. EPC liposome was found to have higher efficiency in inhibiting VSMCs than DPPC. The diameters of EPC and DPPC liposome which encapsulated magnolol became larger than pure EPC and DPPC liposomes. The photos from transmission electron microscopy (TEM) were demonstrated that the EPC and DPPC liposomes could be interfered by magnolol to form a homogeneous liposome. Addition of cholesterol to EPC and DPPC liposome could reduce the liposome diameter.