TY - GEN
T1 - LMP1 gene detection through polymerase chain reaction in microfluidic chip combined with nanoslit surface plasmon resonance sensor
AU - Hsieh, Han Yun
AU - Chang, Ray
AU - Huang, Yung Yu
AU - Wei, Pei Kuen
AU - Fan, Yu Jui
AU - Sheen, Horn Jiunn
N1 - Funding Information:
This paper is supported by the Ministry of Science and Technology in Taiwan, MOST108-2221-E-002-052. REFERENCES [1] M. A. Epstein, B. G. Achong and Y. M. Barr: Virus particles in cultured lymphoblasts from burkitt's lymphoma. Lancet, 1(7335), 702-3 (1964) [2] Y. P. Chen, W. N. Zhang, L. Chen, L. L. Tang, Y. P. Mao, W. F. Li, X. Liu, G. Q. Zhou, Y. Sun, T. B. Kang, M. S. Zeng, N. Liu and J. Ma: Effect of latent membrane protein 1 expression on overall survival in Epstein-Barr virus-associated cancers: a literature-based meta-analysis. Oncotarget, 6(30), 29311-29323 (2015) [3] J. L. Ryan, H. Fan, S. L. Glaser, S. A. Schichman, N. Raab-Traub and M. L. Gulley: Epstein-Barr Virus Quantitation by Real-Time PCR Targeting Multiple Gene Segments: A Novel Approach to Screen for the Virus in Paraffin-Embedded Tissue and Plasma. The Journal of Molecular Diagnostics, 6(4), 378-385 (2004) [4] X.-M. Li, W.-H. Xiao and H.-X. Zhao: Prognostic significance of latent membrane protein 1 expression in non-Hodgkin lymphoma: A meta-analysis. Medicine, 96(14) (2017) CONTACT Han-Yun Hsieh: [email protected]
Publisher Copyright:
© 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.
PY - 2021
Y1 - 2021
N2 - Latent membrane protein 1 (LMP1), one of the main performance proteins of the Epstein-Barr virus (EBV), also highly associated with Nasopharyngeal carcinoma (NPC). Herein, a combined microfluidic polymerase chain reaction (PCR) device with nanoslit surface plasmon resonance (SPR) sensor for LMP1 DNA detection has developed, enabling the amplification and detection of the LMP1 gene on a combined platform. With 30 cycle PCR amplification, the detection limit of 10 pg/mL can be found. Moreover, extracted DNA from EBV-positive cells could be detected through the device, showing the potential of clinical diagnosis. This integrated device performed a label-free, real-time, highly sensitive, low cost, and rapid detection.
AB - Latent membrane protein 1 (LMP1), one of the main performance proteins of the Epstein-Barr virus (EBV), also highly associated with Nasopharyngeal carcinoma (NPC). Herein, a combined microfluidic polymerase chain reaction (PCR) device with nanoslit surface plasmon resonance (SPR) sensor for LMP1 DNA detection has developed, enabling the amplification and detection of the LMP1 gene on a combined platform. With 30 cycle PCR amplification, the detection limit of 10 pg/mL can be found. Moreover, extracted DNA from EBV-positive cells could be detected through the device, showing the potential of clinical diagnosis. This integrated device performed a label-free, real-time, highly sensitive, low cost, and rapid detection.
KW - Epstein-Barr virus (EBV)
KW - microfluidics
KW - polymerase chain reaction
KW - surface plasmon resonance
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M3 - Conference contribution
AN - SCOPUS:85136936883
T3 - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 725
EP - 726
BT - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Y2 - 10 October 2021 through 14 October 2021
ER -