@article{b7f2067f9fda433e81470a75d31ca1d1,
title = "Label-free structural characterization of mitomycin C-modulated wound healing after photorefractive keratectomy by the use of multiphoton microscopy",
abstract = "We applied multiphoton autofluorescence (MAF) and second-harmonic generation (SHG) microscopy to monitor corneal wound healing after photorefractive keratectomy (PRK). Our results show that keratocyte activation can be observed by an increase in its MAF, while SHG imaging of corneal stroma can show the depletion of Bowman's layer after PRK and the reticular collagen deposition in the wound healing stage. Furthermore, quantification of the keratocyte activation and collagen deposition in conjunction with immunohistochemistry and histological images demonstrate the effectiveness of mitomycin C (MMC) in suppressing myofibroblast proliferation and collagen regeneration in the post-PRK wound healing process.",
keywords = "Collagen, Keratocyte activation, Multiphoton autofluorescence excitation, Photorefractive keratectomy, Secondharmonic generation",
author = "Wen Lo and Wang, {Tsung Jen} and Chen, {Wei Liang} and Hsueh, {Chiu Mei} and Chen, {Shean Jen} and Chen, {Yang Fang} and Chou, {Hsiu Chu} and Lin, {Pi Jung} and Hu, {Fung Rong} and Dong, {Chen Yuan}",
note = "Funding Information: We would like to acknowledge the support of the National Science Council of Taiwan, National Taiwan University Medical College, and Taipei Medical University Hospital Research Grant No. 97TMU-TMUH-10. We also acknowledge the Universal Foundation of Medical Service and Health Care Education, Universal Eye Center, and Mr. Chen-Chih Yao for assisting with the experiment.",
year = "2010",
month = may,
doi = "10.1117/1.3432718",
language = "English",
volume = "15",
journal = "Journal of Biomedical Optics",
issn = "1083-3668",
publisher = "SPIE",
number = "3",
}