@article{ef243fa2abd0409aa9535a922276b84a,
title = "Label-free, color-indicating, polarizer-free dye-doped liquid crystal microfluidic polydimethylsiloxane biosensing chips for detecting albumin",
abstract = "We reveal a novel design for dye-doped liquid crystal (DDLC) microfluidic biosensing chips in the polydimethylsiloxane material. With this design chip, the orientation of DDLCs was affected by the interface between the walls of the channels and DDLCs. When the inside of a channel was coated with an N,N-dimethyl-n-octadecyl-3-aminopropyltrimethoxysilyl chloride (DMOAP) alignment layer, the DDLCs oriented homeotropically in a homeotropic (H) state under cross-polarized microscopy. After immobilization of antigens with antibodies on the alignment layer-coated microchannel walls, the optical intensity of the DDLC change from the dark H state to the bright planar (P) state. Using pressure-driven flow, the binding of antigens/antibodies to the DDLCs could be detected in an experimental sequential order. The microfluidic DDLCs were tested by detecting bovine serum albumin (BSA) and its immune-responses of antigens/antibodies. We proved that this immunoassay chip was able to detect BSA antigens/antibodies pairs with the detection limit about 0.5 µg/mL. The novel DDLC chip was shown to be a simple, multi-detection device, and label-free microfluidic chips are presented.",
keywords = "Albumin, Biosensing chips, Dye-doped liquid crystal, Microfluidic",
author = "Chen, {Fu Lun} and Luh, {Hui Tzung} and Hsiao, {Yu Cheng}",
note = "Funding Information: Figure 6. Intensities of dye-doped liquid crystal (DDLC)biosensor chips at various bovine serum albumin (BSA) with a 1 µg/mL of BSA antibody. Figure 6. albumin (BSA)Intensitiewith a 1s of dye-dµg/mL of oped liquBSA antiboid crystal (DDLC)dy. biosensor chips at various bovine serum albumin (BSA) with a 1 µg/mL of BSA antibody. 4. ConTheclusDDLCions microfluidic biosensing chips are presented in this study. The orientations 4. ConTclhues DioDnLsC microfluid ic biosensingchips are presentedin this study. The orientations of DDLCs are affected by the sensitive interface effectbetween themicrochannels and a biomolecule. The DDLCs were initiallyDMOalignedAP alignment layer is vertically and exhibitedalso acobrightated insideH statethundere mictherochannon-nel. The of DDLCs are affected by the sensitive interface effectbetween themicrochannels and a biomolecule. The DMOAP alignment layer is also coated inside the microchannel. The DDbeLcCasu sweeofrteh ieniinttiearlrlyupatliiognniendt hveerdtiirceactliloynaonfdDeDxLhCib.iUtesidngapbrreisgshutreH-d sritvaeten uflnowde,rthtehBeSnAon-po - larainzteidg emn/icanrotisbcoodpyyi. mAmftuenr etchoem BpSlAex easnctaignebnesd heatedctbedoubnydm tioc rtohsec oBpSyA. Inaandtidbiotidoine, sininthethe mi - bDeDcaLuCsed oefv tihce,et ihneteimrrumputnioondetienc tthioen dliirmecittioofnB SoAf DanDtiLgCen. /Uansitnibgo dpyreisssu0.r0e1-dµrg/ivmeLn oflfoBwSA, the BS A aanntdig1enµ/g/anmtLiboofdayn tiim-BmSAu.nWe ecopmropvleedxeths at tchaisnm biec rdoefltueidctiecdDDbLyCmimicrmousncooapsysa. yInbioasdednistiinogn, in the DDLC device,the immunodetection limit of BSA antigen/antibodyis 0.01µg/mL of BSA and 1 µg/mL of anti-BSA. We proved thatthis microfluidic DDLCimmunoassay biosens-a sensitive, inexpensive, multi-detection, color indicating and non-polarizer system for and 1 µg/mL of anti-BSA. We proved thatthis microfluidic DDLCimmunoassay biosens-DDLC-based immunoassays. free DDLC immunoassay chip. The new design of this DDLC biosensing chip provides a sensitive, inexpensive, multi-detection, color indicating andnon-polarizer system for sensitive, inexpensive, multi-detection, color indicating andnon-polarizer system for DDLC-bato the publishedsed immunoassays. version of the manuscript. Funding: This work was financially supported by the Ministry of Science and Technology, Taiwan, tion, F.-L.C.;Project administration, H.-T.L.;Writing,Y.-C.H. and H.-T.L.Allauthors have readand tionagreed to the, F.-L.C.; Project apublished dminversioistrationn of the manuscript. , H.-T.L.; Writing, Y.-C.H. and H.-T.L. All authors have read and Funding Information: Funding:Thiswork was financiallysupported bytheMinistry ofScienceand Technology, Taiwan, under grant no. MOST 109-2636-E-038-001 and Taipei Medical University-Wan Fang Hospital, Tai-under grant no. MOST 109-2636-E-038-001 and Taipei Medical University-Wan Fang Hospital, Taiwan under grant no. 110TMU-WFH-15. Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",
year = "2021",
month = aug,
day = "2",
doi = "10.3390/polym13162587",
language = "English",
volume = "13",
journal = "Polymers",
issn = "2073-4360",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "16",
}