TY - JOUR
T1 - Iron and phosphate content of rat ferritin heteropolymers
AU - Juan, Shu Hui
AU - Aust, Steven D.
N1 - Funding Information:
We gratefully acknowledge the technical assistance of Randy Booth and Ryan Berger. We also thank T. Maughan for her secretarial assistance in the preparation the manuscript. The work was supported by NIH Grant ES05056.
PY - 1998/9/15
Y1 - 1998/9/15
N2 - An attempt was made to relate the iron and phosphate content of ferritin to its subunit composition. Ferritins from various tissues were separated according to their subunit composition by anion exchange chromatography and according to their iron content by density-gradient centrifugation. Iron and phosphate contents were not related to subunit composition. Recombinant rat liver ferritin heteropolymers of different subunit composition (1, 4, 6, 10, 15, and 17 H chains per 24 mer) were maximally loaded with iron, using ceruloplasmin and phosphate. All loaded approximately the same amount of iron and phosphate (2250 and 380 atoms, respectively). The iron and phosphate content of all ferritin, including the maximally loaded recombinant ferritin heteropolymers, fit an equation we previously reported: [Fe] = 4404 - 5.61 [Pi] (D. deSilva et al., 1993, Arch. Biochem. Biophys. 303, 451-455). These results suggest that the amount of iron and apparently the space within the core of ferritin were not related to different subunit composition.
AB - An attempt was made to relate the iron and phosphate content of ferritin to its subunit composition. Ferritins from various tissues were separated according to their subunit composition by anion exchange chromatography and according to their iron content by density-gradient centrifugation. Iron and phosphate contents were not related to subunit composition. Recombinant rat liver ferritin heteropolymers of different subunit composition (1, 4, 6, 10, 15, and 17 H chains per 24 mer) were maximally loaded with iron, using ceruloplasmin and phosphate. All loaded approximately the same amount of iron and phosphate (2250 and 380 atoms, respectively). The iron and phosphate content of all ferritin, including the maximally loaded recombinant ferritin heteropolymers, fit an equation we previously reported: [Fe] = 4404 - 5.61 [Pi] (D. deSilva et al., 1993, Arch. Biochem. Biophys. 303, 451-455). These results suggest that the amount of iron and apparently the space within the core of ferritin were not related to different subunit composition.
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U2 - 10.1006/abbi.1998.0847
DO - 10.1006/abbi.1998.0847
M3 - Article
C2 - 9735170
AN - SCOPUS:0032530144
SN - 0003-9861
VL - 357
SP - 293
EP - 298
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
IS - 2
ER -