Involvement of phosphatidylcholine-phospholipase C and protein kinase C in peptidoglycan-induced nuclear factor-κB activation and cyclooxygenase-2 expression in RAW 264.7 macrophages

Jam Inn Tzeng; Bing Chang Chen; Huey Mei Chang; Jhi Joung Wang; Munisamy Sureshbabu; Ming Hsien Chien; Ming Jen Hsu; Mauo Ying Bien; Wen Ta Chiu; Chuang Ye Hong; Chien Huang Lin

doi:10.1016/j.phrs.2009.09.005

Involvement of phosphatidylcholine-phospholipase C and protein kinase C in peptidoglycan-induced nuclear factor-κB activation and cyclooxygenase-2 expression in RAW 264.7 macrophages

Jam Inn Tzeng, Bing Chang Chen, Huey Mei Chang, Jhi Joung Wang, Munisamy Sureshbabu, Ming Hsien Chien, Ming Jen Hsu, Mauo Ying Bien, Wen Ta Chiu, Chuang Ye Hong, Chien Huang Lin

研究成果: 雜誌貢獻 › 文章 › 同行評審

16 引文斯高帕斯（Scopus）

摘要

In this study, we examined the role of phosphatidylcholine-phospholipase C (PC-PLC) and protein kinase C (PKC) in peptidoglycan (PGN)-induced nuclear factor-κB (NF-κB) activation and cyclooxygenase-2 (COX-2) expression in RAW 264.7 macrophages. PGN-induced COX-2 expression was attenuated by a PC-PLC inhibitor (D609) and by PKC inhibitors (Go 6976 and Ro 31-8220), but not by a phosphatidylinositol-PLC (PI-PLC) inhibitor (U-73122). PGN caused an increase in PKC activity, and this effect was inhibited by D609, Go 6976, and Ro 31-8220, but not by U-73122. Furthermore, the PGN-mediated increases in κB-luciferase activity were also inhibited by D609 and Ro 31-8220. Our data demonstrate that PGN activates PC-PLC which induces PKC activation; this in turn initiates NF-κB activation, and ultimately induces COX-2 expression in RAW 264.7 macrophages.

原文	英語
頁（從 - 到）	162-166
頁數	5
期刊	Pharmacological Research
卷	61
發行號	2
DOIs	https://doi.org/10.1016/j.phrs.2009.09.005
出版狀態	已發佈 - 2月 2010

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10.1016/j.phrs.2009.09.005

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Tzeng, J. I., Chen, B. C., Chang, H. M., Wang, J. J., Sureshbabu, M., Chien, M. H., Hsu, M. J., Bien, M. Y., Chiu, W. T., Hong, C. Y., & Lin, C. H. (2010). Involvement of phosphatidylcholine-phospholipase C and protein kinase C in peptidoglycan-induced nuclear factor-κB activation and cyclooxygenase-2 expression in RAW 264.7 macrophages. Pharmacological Research, 61(2), 162-166. https://doi.org/10.1016/j.phrs.2009.09.005

Tzeng, JI, Chen, BC, Chang, HM, Wang, JJ, Sureshbabu, M, Chien, MH , Hsu, MJ, Bien, MY, Chiu, WT , Hong, CY & Lin, CH 2010, 'Involvement of phosphatidylcholine-phospholipase C and protein kinase C in peptidoglycan-induced nuclear factor-κB activation and cyclooxygenase-2 expression in RAW 264.7 macrophages', Pharmacological Research, 卷 61, 編號 2, 頁 162-166. https://doi.org/10.1016/j.phrs.2009.09.005

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title = "Involvement of phosphatidylcholine-phospholipase C and protein kinase C in peptidoglycan-induced nuclear factor-κB activation and cyclooxygenase-2 expression in RAW 264.7 macrophages",

abstract = "In this study, we examined the role of phosphatidylcholine-phospholipase C (PC-PLC) and protein kinase C (PKC) in peptidoglycan (PGN)-induced nuclear factor-κB (NF-κB) activation and cyclooxygenase-2 (COX-2) expression in RAW 264.7 macrophages. PGN-induced COX-2 expression was attenuated by a PC-PLC inhibitor (D609) and by PKC inhibitors (Go 6976 and Ro 31-8220), but not by a phosphatidylinositol-PLC (PI-PLC) inhibitor (U-73122). PGN caused an increase in PKC activity, and this effect was inhibited by D609, Go 6976, and Ro 31-8220, but not by U-73122. Furthermore, the PGN-mediated increases in κB-luciferase activity were also inhibited by D609 and Ro 31-8220. Our data demonstrate that PGN activates PC-PLC which induces PKC activation; this in turn initiates NF-κB activation, and ultimately induces COX-2 expression in RAW 264.7 macrophages.",

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author = "Tzeng, {Jam Inn} and Chen, {Bing Chang} and Chang, {Huey Mei} and Wang, {Jhi Joung} and Munisamy Sureshbabu and Chien, {Ming Hsien} and Hsu, {Ming Jen} and Bien, {Mauo Ying} and Chiu, {Wen Ta} and Hong, {Chuang Ye} and Lin, {Chien Huang}",

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T1 - Involvement of phosphatidylcholine-phospholipase C and protein kinase C in peptidoglycan-induced nuclear factor-κB activation and cyclooxygenase-2 expression in RAW 264.7 macrophages

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AU - Chang, Huey Mei

AU - Wang, Jhi Joung

AU - Sureshbabu, Munisamy

AU - Chien, Ming Hsien

AU - Hsu, Ming Jen

AU - Bien, Mauo Ying

AU - Chiu, Wen Ta

AU - Hong, Chuang Ye

AU - Lin, Chien Huang

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N2 - In this study, we examined the role of phosphatidylcholine-phospholipase C (PC-PLC) and protein kinase C (PKC) in peptidoglycan (PGN)-induced nuclear factor-κB (NF-κB) activation and cyclooxygenase-2 (COX-2) expression in RAW 264.7 macrophages. PGN-induced COX-2 expression was attenuated by a PC-PLC inhibitor (D609) and by PKC inhibitors (Go 6976 and Ro 31-8220), but not by a phosphatidylinositol-PLC (PI-PLC) inhibitor (U-73122). PGN caused an increase in PKC activity, and this effect was inhibited by D609, Go 6976, and Ro 31-8220, but not by U-73122. Furthermore, the PGN-mediated increases in κB-luciferase activity were also inhibited by D609 and Ro 31-8220. Our data demonstrate that PGN activates PC-PLC which induces PKC activation; this in turn initiates NF-κB activation, and ultimately induces COX-2 expression in RAW 264.7 macrophages.

AB - In this study, we examined the role of phosphatidylcholine-phospholipase C (PC-PLC) and protein kinase C (PKC) in peptidoglycan (PGN)-induced nuclear factor-κB (NF-κB) activation and cyclooxygenase-2 (COX-2) expression in RAW 264.7 macrophages. PGN-induced COX-2 expression was attenuated by a PC-PLC inhibitor (D609) and by PKC inhibitors (Go 6976 and Ro 31-8220), but not by a phosphatidylinositol-PLC (PI-PLC) inhibitor (U-73122). PGN caused an increase in PKC activity, and this effect was inhibited by D609, Go 6976, and Ro 31-8220, but not by U-73122. Furthermore, the PGN-mediated increases in κB-luciferase activity were also inhibited by D609 and Ro 31-8220. Our data demonstrate that PGN activates PC-PLC which induces PKC activation; this in turn initiates NF-κB activation, and ultimately induces COX-2 expression in RAW 264.7 macrophages.

KW - COX-2

KW - Inflammation

KW - NF-κB

KW - Peptidoglycan

KW - Protein kinase C

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Involvement of phosphatidylcholine-phospholipase C and protein kinase C in peptidoglycan-induced nuclear factor-κB activation and cyclooxygenase-2 expression in RAW 264.7 macrophages

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