Intracellular oxidative cleavage of DNA in escherichia coli by the copper-1, 10-phenanthroline complex

Eleonore Lickl; Shiow Chyi Chao; Wen Chang Chang

doi:10.3109/10715768909087970

Intracellular oxidative cleavage of DNA in escherichia coli by the copper-1, 10-phenanthroline complex

Eleonore Lickl, Shiow Chyi Chao, Wen Chang Chang

研究成果: 雜誌貢獻 › 文章 › 同行評審

9 引文斯高帕斯（Scopus）

摘要

Plasmid and chromosomal DNA of E. coli during exponential growth are cleaved after treatment with copper(II)-1, 10-phenanthroline complex (1: 2) without providing any exogenous reductant. About 1500 copper molecules per cell are present as estimated by atomic absorption analysis. Within the cell the endogenous reducing substances may have participated in the sequential oxidative reactions, which lead to the damage of DNA. A portion of the resultant DNA fragments originates from plasmid DNA as demonstrated by hybridization tests.

原文	英語
頁（從 - 到）	37-45
頁數	9
期刊	Free Radical Research
卷	8
發行號	1
DOIs	https://doi.org/10.3109/10715768909087970
出版狀態	已發佈 - 1月 1 1989
對外發佈	是

ASJC Scopus subject areas

生物化學

存取文件

10.3109/10715768909087970

其它文件與鏈接

Link to publication in Scopus

引用此

@article{98d0904de9b34aa78c8d7c806db007b4,

title = "Intracellular oxidative cleavage of DNA in escherichia coli by the copper-1, 10-phenanthroline complex",

abstract = "Plasmid and chromosomal DNA of E. coli during exponential growth are cleaved after treatment with copper(II)-1, 10-phenanthroline complex (1: 2) without providing any exogenous reductant. About 1500 copper molecules per cell are present as estimated by atomic absorption analysis. Within the cell the endogenous reducing substances may have participated in the sequential oxidative reactions, which lead to the damage of DNA. A portion of the resultant DNA fragments originates from plasmid DNA as demonstrated by hybridization tests.",

keywords = "Copper-phenanthroline, DNA damage, E. coli, In vivo model",

author = "Eleonore Lickl and Chao, {Shiow Chyi} and Chang, {Wen Chang}",

year = "1989",

month = jan,

day = "1",

doi = "10.3109/10715768909087970",

language = "English",

volume = "8",

pages = "37--45",

journal = "Free Radical Research",

issn = "1071-5762",

publisher = "Informa Healthcare",

number = "1",

}

TY - JOUR

T1 - Intracellular oxidative cleavage of DNA in escherichia coli by the copper-1, 10-phenanthroline complex

AU - Lickl, Eleonore

AU - Chao, Shiow Chyi

AU - Chang, Wen Chang

PY - 1989/1/1

Y1 - 1989/1/1

N2 - Plasmid and chromosomal DNA of E. coli during exponential growth are cleaved after treatment with copper(II)-1, 10-phenanthroline complex (1: 2) without providing any exogenous reductant. About 1500 copper molecules per cell are present as estimated by atomic absorption analysis. Within the cell the endogenous reducing substances may have participated in the sequential oxidative reactions, which lead to the damage of DNA. A portion of the resultant DNA fragments originates from plasmid DNA as demonstrated by hybridization tests.

AB - Plasmid and chromosomal DNA of E. coli during exponential growth are cleaved after treatment with copper(II)-1, 10-phenanthroline complex (1: 2) without providing any exogenous reductant. About 1500 copper molecules per cell are present as estimated by atomic absorption analysis. Within the cell the endogenous reducing substances may have participated in the sequential oxidative reactions, which lead to the damage of DNA. A portion of the resultant DNA fragments originates from plasmid DNA as demonstrated by hybridization tests.

KW - Copper-phenanthroline

KW - DNA damage

KW - E. coli

KW - In vivo model

UR - http://www.scopus.com/inward/record.url?scp=0024790775&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024790775&partnerID=8YFLogxK

U2 - 10.3109/10715768909087970

DO - 10.3109/10715768909087970

M3 - Article

C2 - 2555284

AN - SCOPUS:0024790775

SN - 1071-5762

VL - 8

SP - 37

EP - 45

JO - Free Radical Research

JF - Free Radical Research

IS - 1

ER -

Intracellular oxidative cleavage of DNA in escherichia coli by the copper-1, 10-phenanthroline complex

摘要

ASJC Scopus subject areas

存取文件

其它文件與鏈接

指紋

引用此