Intracellular acidification enhances neutrophil phagocytosis in chronic haemodialysis patients: Possible role of CD11b/CD18

Chien Sheng Lin, Joseph G. Wann, Cheng Wen Hsiao, Deborah W. Tai, Jin Shuen Chen, Yung Ho Hsu, Huei Chen Huang, Chung Faye Chao

研究成果: 雜誌貢獻文章同行評審

4 引文 斯高帕斯(Scopus)


Background. We have demonstrated that uraemic neutrophils that exhibit a low intracellular pH (pHi) display enhanced phagocytosis. However, the underlying cellular mechanism is unclear. Methods. We used neutrophils from three groups of haemodialysis (HD) patients before dialysis (Groups A, B and C) and also from age- and sex-matched healthy individuals to determine pHi, phagocytosis and expression of CD11b, CD18, CD14 and toll-like receptors (TLR)-2 and TLR-4. The patients were categorized based on three consecutive monthly pre-dialysis plasma bicarbonate concentrations(PHCO3) and pH values; Groups A, B and C had a constant pre-dialysis PHCO3 of ≤21, 21-26 and ≥26 mmol/L (mEq/L), respectively. We also studied the effects induced by the correction of metabolic acidosis and monoclonal antibodies (mAbs) against CD11b/CD18 on neutrophils in Group A. Furthermore, we investigated the effect of intracellular acidification on uraemic neutrophils ex vivo. Results. We observed that the neutrophils in Group A exhibited significantly increased phagocytosis and expression of CD11b/CD18 compared with those in Groups B and C. Additionally, our ex vivo studies demonstrated that the mAbs against CD11b/CD18 partially blocked the enhancement of neutrophil phagocytosis in Group A. Moreover, the pHi of uraemic neutrophils is inversely correlated with phagocytosis and expression of CD11b/CD18. Conclusions. HD patients with a low PHCO3 exhibited low neutrophil pHi that in turn increased the expression of CD11b/CD18 compared with neutrophils with a normal or high pHi. This increased expression of CD11b/CD18 on the uraemic neutrophils may contribute to the pHi-mediated phagocytosis.

頁(從 - 到)1642-1649
期刊Nephrology Dialysis Transplantation
出版狀態已發佈 - 5月 2008

ASJC Scopus subject areas

  • 腎臟病學
  • 移植


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