TY - JOUR
T1 - Intercomparison of conventional and QuickScan dicentric scoring for the validation of individual biodosimetry analysis in Taiwan
AU - Lin, Wan Chi
AU - Chang, Kang Wei
AU - Liao, Tse Zung
AU - Ou Yang, Fang Yu
AU - Chang, Tsui Jung
AU - Yuan, Ming Chen
AU - Wilkins, Ruth C.
AU - Chang, Chih Hsien
N1 - Funding Information:
We thank Dr. Ingrid Y. Liu from Tzu Chi University for assistant of Biodosimetry laboratory establishment, Dr. Cheng-Fu Chao and Mr. Bin Lin for technical assistant and National Radiation Standard Laboratory of Taiwan for irradiation of blood samples. We also thank for the support of project by Atomic Energy Council (AEC), Executive Yuan, Taiwan.
Funding Information:
We thank Dr. Ingrid Y. Liu from Tzu Chi University for assistant of Biodosimetry laboratory establishment, Dr. Cheng-Fu Chao and Mr. Bin Lin for technical assistant and National Radiation Standard Laboratory of Taiwan for irradiation of blood samples. We also thank for the support of project by Atomic Energy Council (AEC), Executive Yuan, Taiwan.
Publisher Copyright:
© Copyright © 2021 Taylor & Francis Group LLC.
PY - 2021
Y1 - 2021
N2 - Purpose: The dicentric chromosome assay (DCA), the gold standard for radiation biodosimetry, evaluates an individual absorbed radiation dose by the analysis of DNA damage in human lymphocytes. The conventional (C-DCA) and QuickScan (QS-DCA) scoring methods are sensitive for estimating radiation dose. The Biodosimetry Laboratory at Institute of Nuclear Energy Research (INER), Taiwan, participated in intercomparison exercises conducted by Health Canada (HC) in 2014, 2015 and 2018 to validate the laboratory’s accuracy and performance. Material and methods: Blood samples for the conventional dose response curve for Taiwan were irradiated with 0, 0.25, 0.5, 1, 2, 3, 4 and 5 Gy. Ten blind blood samples were provided by HC. Either or both of two methods of conventional (C) or QuickScan (QS) scoring could be chosen for the HC’s intercomparison. For C-DCA triage scoring, only cells with 46 centromeres were counted and each scorer recorded the number of dicentrics in the first 50 metaphases or stopped scoring when 30 dicentrics were reached. Scorers also recorded how much time it took to analyze 10, 20, and 50 cells. Subsequently, the data were entered into the Dose Estimate software (DoseEstimate_v5.1) and dose estimates were calculated. With QS-DCA scoring, a minimum of 50 metaphase cells (or 30 dicentrics) were scored in apparently complete metaphases without verification of exactly 46 centromeres. Results: For the blinded blood samples irradiated at HC and shipped to INER, the mean absolute deviation (MAD) derived after scoring 50 cells for C-DCA and QS-DCA was <0.5 Gy for all three intercomparisons, meeting the criteria for acceptance. Conclusion: The results indicated that the Biodosimetry Laboratory at INER can provide reliable dose estimates in the case of a large-scale radiation accident.
AB - Purpose: The dicentric chromosome assay (DCA), the gold standard for radiation biodosimetry, evaluates an individual absorbed radiation dose by the analysis of DNA damage in human lymphocytes. The conventional (C-DCA) and QuickScan (QS-DCA) scoring methods are sensitive for estimating radiation dose. The Biodosimetry Laboratory at Institute of Nuclear Energy Research (INER), Taiwan, participated in intercomparison exercises conducted by Health Canada (HC) in 2014, 2015 and 2018 to validate the laboratory’s accuracy and performance. Material and methods: Blood samples for the conventional dose response curve for Taiwan were irradiated with 0, 0.25, 0.5, 1, 2, 3, 4 and 5 Gy. Ten blind blood samples were provided by HC. Either or both of two methods of conventional (C) or QuickScan (QS) scoring could be chosen for the HC’s intercomparison. For C-DCA triage scoring, only cells with 46 centromeres were counted and each scorer recorded the number of dicentrics in the first 50 metaphases or stopped scoring when 30 dicentrics were reached. Scorers also recorded how much time it took to analyze 10, 20, and 50 cells. Subsequently, the data were entered into the Dose Estimate software (DoseEstimate_v5.1) and dose estimates were calculated. With QS-DCA scoring, a minimum of 50 metaphase cells (or 30 dicentrics) were scored in apparently complete metaphases without verification of exactly 46 centromeres. Results: For the blinded blood samples irradiated at HC and shipped to INER, the mean absolute deviation (MAD) derived after scoring 50 cells for C-DCA and QS-DCA was <0.5 Gy for all three intercomparisons, meeting the criteria for acceptance. Conclusion: The results indicated that the Biodosimetry Laboratory at INER can provide reliable dose estimates in the case of a large-scale radiation accident.
KW - Biodosimetry
KW - dicentric chromosome assay
KW - intercomparison exercises
KW - radiation accident
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U2 - 10.1080/09553002.2021.1928789
DO - 10.1080/09553002.2021.1928789
M3 - Article
C2 - 34003708
AN - SCOPUS:85107442405
SN - 0955-3002
VL - 97
SP - 916
EP - 925
JO - International Journal of Radiation Biology
JF - International Journal of Radiation Biology
IS - 7
ER -