TY - JOUR
T1 - Interaction between the left-handed Z-DNA and polyamine-2
T2 - The crystal structure of the d(CG)3 and spermidine complex
AU - Ohishi, Hirofumi
AU - Nakanishi, Isao
AU - Inubushi, Kouichi
AU - Van Der Marel, Gijs
AU - Van Boom, Jacques H.
AU - Rich, Alexander
AU - Wang, Andrew H.J.
AU - Hakoshima, Toshio
AU - Tomita, Ken Ichi
PY - 1996/8/5
Y1 - 1996/8/5
N2 - This paper deals with the crystal structure of d(CG)3-spermidine complex. The DNA fragment, d(CG)3, was crystallized with N-(2-amino-propyl)- 1,4-diamino-butane, PA(34), spermidine. The results of its X-ray crystallographic analysis showed many intermolecular contacts between d(CG)3 and spermidine, but the binding mode of spermidine to the d(CG)3 molecule is different from that of the d(CG)3 and N-(2-amino-ethyl)-1,4-diamino-butane [PA(24)] complex: a spermidine molecule bound to the d(CG)3 and its symmetrically related neighboring d(CG)3 molecules through the water molecules with hydrogen bonds, while one PA(24) molecule connected directly to one d(CG)3 molecule, but not to its neighboring d(CG)3 molecule. In the crystal, the d(CG)3 molecule was the left-handed Z-form, and three magnesium cations and a sodium cation were observed around the d(CG)3 moiety with different binding modes from the case of the d(CG)3-PA(24) complex.
AB - This paper deals with the crystal structure of d(CG)3-spermidine complex. The DNA fragment, d(CG)3, was crystallized with N-(2-amino-propyl)- 1,4-diamino-butane, PA(34), spermidine. The results of its X-ray crystallographic analysis showed many intermolecular contacts between d(CG)3 and spermidine, but the binding mode of spermidine to the d(CG)3 molecule is different from that of the d(CG)3 and N-(2-amino-ethyl)-1,4-diamino-butane [PA(24)] complex: a spermidine molecule bound to the d(CG)3 and its symmetrically related neighboring d(CG)3 molecules through the water molecules with hydrogen bonds, while one PA(24) molecule connected directly to one d(CG)3 molecule, but not to its neighboring d(CG)3 molecule. In the crystal, the d(CG)3 molecule was the left-handed Z-form, and three magnesium cations and a sodium cation were observed around the d(CG)3 moiety with different binding modes from the case of the d(CG)3-PA(24) complex.
KW - Crystal structure
KW - Molecular complex
KW - Molecular interaction
KW - Polyamine
KW - Spermidine
KW - Z-DNA
KW - d(CG)
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U2 - 10.1016/0014-5793(96)00723-5
DO - 10.1016/0014-5793(96)00723-5
M3 - Article
C2 - 8706905
AN - SCOPUS:0030570768
SN - 0014-5793
VL - 391
SP - 153
EP - 156
JO - FEBS Letters
JF - FEBS Letters
IS - 1-2
ER -