Inhibitory effects of terpenoids from the fermented broth of the ascomycete stilbohypoxylon elaeicola YMJ173 on nitric oxide production in RAW264.7 macrophages

A series of six isopimarane-type diterpene glycosides, along with an eremophilane-type sesquiterpene, i.e., elaeicolasides A-C (1-3, resp.), 16-(α-D-mannopyranosyloxy)isopimar-7-en-19-oic acid (4), hymatoxin K (5), hymatoxin L (6), and elaeicolalactone (7), were isolated from the AcOEt extract of the fermented broth of Stilbohypoxylon elaeicola YMJ173. Among these, 1-3 and 7 are new compounds based on their spectroscopic data and sugar composition analysis. The effects of 1-7 on the inhibition of nitric oxide (NO) production in lipopolysaccharide (LPS)-activated murine macrophage RAW264.7 cells were evaluated. All these compounds inhibited NO production, detected as nitrite in the culture medium, in activated macrophages without any cytotoxicity at a concentration of 100μM. Among these compounds, 2 showed a significant activity with the average maximum inhibition (E _max) and median inhibitory concentration (IC ₅₀) values of 93.3±0.5% and 79.3±0.4μM, respectively.