Inhibition of xanthine oxidase by synthetic cytokinin analogues

Shiow Yunn Sheu, Yu Chan Lin, Hsüch Ching Chiang

研究成果: 雜誌貢獻文章同行評審

7 引文 斯高帕斯(Scopus)


Thirteen synthetic cytokinin analogues were tested for their inhibitory effects on xanthine oxidase. The enzyme, xanthine oxidase catalyses the oxidation of hypoxanthine to xanthine and of xanthine to uric acid, which has a λmax of 295 nm, forming the basis for a spectrophotometric assay of the activity of xanthine oxidase. The results showed that 8-azaadenine(1), 4-amino-6-hydroxypyrazolo [3,4-d] pyrimidine(4), 4-amino-6-mercaptopyrazolo [3,4-d] pyrimidine(5) and 4-aminopyrazolo [3,4-d] pyrimidine(6) display inhibitory effects on xanthine oxidase with an order of activity of IC50 = 0.54, 5.91, 8.17 and 25.46 μM, respectively. Their apparent inhibition constants (Ki) were 0.66, 1.54, 6.61 and 26.79 μM, and induced mixed(competitive-non-competitive), competitive, mixed (competitive-non-competitive), and competitive types of inhibition respectively, with respect to the substrate xanthine.

頁(從 - 到)1043-1049
期刊Anticancer Research
發行號2 A
出版狀態已發佈 - 1997

ASJC Scopus subject areas

  • 癌症研究
  • 腫瘤科


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