Inhibition of xanthine oxidase by benzothiazinone analogues

Shiow Yunn Sheu, Chun Yu Lin, Jen Der Wu, Hsüch Ching Chiang

研究成果: 雜誌貢獻文章同行評審

9 引文 斯高帕斯(Scopus)


Fourteen synthetic benzothiazinone analogues were tested for their inhibitory effects on xanthine oxidase. The enzyme, xanthine oxidase (XO) catalyses the oxidation of hypoxanthine to xanthine and of xanthine to uric acid, which has a λmax of 295 nm, forming the basis for a spectrophotometric assay for the activity of xanthine oxidase. The results showed that 2-amino-4H-1,3-benzothiazine-4-one (1), 2-guanidino-4H-1,3-benzothiazin-4-one (2) and rhodanine (3) display inhibitory effects on xanthine oxidase with an order of activity of IC-50 = 5.54, 5.60 and 121.40 μM respectively. The apparent inhibition constants (Ki) were 5.12, 19.47 and 206.23 μM, and induced non-competitive, mixed type (non-competitive-uncompetitive) and mixed type (non-competitive-uncompetitive) inhibitions respectively with respect to the substrate xanthine.

頁(從 - 到)119-123
期刊Anticancer Research
發行號1 A
出版狀態已發佈 - 1999

ASJC Scopus subject areas

  • 癌症研究
  • 腫瘤科


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