摘要
The effect of mouse recombinant interferon-gamma (rIFN-γ) on murine lymphokine-activated killer (LAK) cell activity was investigated using natural killer (NK)-resistant, spontaneously developed, weakly immunogenic and highly tumourigenic, syngeneic murine mammary adenocarcinoma, JC, mimicking that of human disease, as the target. Murine YAC-1 also was used as a target cell line. rIFN-γ, when used in combination with recombinant interleukin-2 (rIL-2), was shown to exhibit a suppressed effect on LAK cell activity generated from BALB/c mouse splenocytes, compared to that with rIL-2 alone. The decrease in LAK cell activity was rIFN-γ dose-dependent. Kinetic study revealed that this inhibitory effect was demonstrated when rIFN-γ was added to the medium at the early phase of rIL-2 culture. The inhibitory effect on LAK cell generation by rIFN-γ was completely abrogated when the nylon-wool-treated non-adherent 'macrophage-free' splenocytes were incubated with rIL-2 and rIFN-γ. These results indicated that the LAK cell activity generated from murine splenocytes cultured with rIL-2 could be depressed by rIFN-γ, and that the macrophages may be involved as mediators.
原文 | 英語 |
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頁(從 - 到) | 116-120 |
頁數 | 5 |
期刊 | Immunology |
卷 | 70 |
發行號 | 1 |
出版狀態 | 已發佈 - 1990 |
對外發佈 | 是 |
ASJC Scopus subject areas
- 免疫學和過敏
- 免疫學