In vitro evaluation of intracellular activity of antibiotics against non-typhoid Salmonella

Cheng Hsun Chiu, Tzou Yien Lin, Jonathan T. Ou

研究成果: 雜誌貢獻文章同行評審

33 引文 斯高帕斯(Scopus)


Non-typhoid salmonellae are the most common causative organisms of bacterial enteritis in children. Clinical studies have failed to show any influence of various antibiotics on the natural course of acute salmonella enteritis. Poor penetration of antibiotics into phagocytic cells that contain intracellular Salmonella spp., and possible intracellular antibiotic inactivation have been considered as possible reasons for this. In this study, we used an in vitro model to assess the intracellular activity of antibiotics against non-typhoid salmonellae. The survival of intracellular Salmonella spp. in P388D1 cells, a mouse macrophage cell line was measured in the presence of various antibiotics. Except for gentamicin, which entered phagocytes poorly, ofloxacin, azithromycin, chloramphenicol and three β-lactam antibiotics, ampicillin, cefixime and ceftriaxone, exhibited bacteriostatic activity against susceptible intracellular Salmonella spp. at an extracellular concentration equal to the minimal inhibitory concentration (MIC). At a concentration of 10xMIC, neither chloramphenicol nor the three β-lactam antibiotics produced a bactericidal response; however, both ofloxacin and azithromycin were bactericidal after 8-24 h of incubation. The results showed that fluoroquinolones and new macrolides were more efficient than the other antibiotics in eradicating intracellular salmonella and might be useful agents for the treatment of non-typhoid salmonella enteritis in children. Clinical trials should be considered. Copyright (C) 1999 Elsevier Science B.V.
頁(從 - 到)47-52
期刊International Journal of Antimicrobial Agents
出版狀態已發佈 - 6月 1999

ASJC Scopus subject areas

  • 微生物學(醫學)
  • 藥學(醫學)
  • 傳染性疾病


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