@article{a367cc57093c45d7984efcf3fa284ca4,
title = "In Vitro Antimicrobial Potential of CAPE and Caffeamide Derivatives against Oral Microbes",
abstract = "Caffeic acid phenethyl ester (CAPE) is a natural component isolated from propolis and used in traditional medicine. We aimed to investigate the antimicrobial properties and action mechanism of CAPE and caffeamide derivatives (26G and 36M) against oral disease microbes. We resolved the minimum inhibitory and bactericidal concentrations of 26G and 36M and their stability at different temperatures and pH. We also evaluated their effect on biofilm formation and antibiotic resistance gene expression in methicillin-resistant Staphylococcus aureus (MRSA). Our results revealed that 26G and 36M showed the best anticancer and antimicrobial activities, respectively, compared with the other four caffeamide derivatives. Both 26G and 36M showed heat-dependent decreases in antimicrobial activity. The 36M derivative was stable irrespective of pH, whereas 26G was not stable under high pH conditions. Biofilm formation and antibiotic resistance-related gene expression were consistent with their respective phenotypes. This study provides evidence for the potential application of CAPE and caffeamide derivatives in dental medicine to cure or prevent oral diseases.",
keywords = "antibiotic resistance, biofilm, caffeamide, caffeic acid phenethyl ester (CAPE), minimum bactericidal concentration, minimum inhibitory concentration",
author = "Shih, {Yin Hwa} and Hsia, {Shih Min} and Chiu, {Kuo Chou} and Wang, {Tong Hong} and Chien, {Chi Ying} and Li, {Po Jung} and Kuo, {Yueh Hsiung} and Shieh, {Tzong Ming}",
note = "Funding Information: Funding: This research was funded by the Ministry of Science and Technology, Taiwan, grant numbers MOST 108-2314-B-039-009-MY3; the China Medical University, Taiwan, grant number CMU110-MF-31, CMU110-S-43, CMU110-Z-08, CMU109-AWARD-02; and “Chinese Medicine Research Center, China Medical University” from The Featured Areas Research Center Program within the framework of the Higher Education Sprout Project by the Ministry of Education (MOE) in Taiwan (CMRC-CHM-2-1). Funding Information: This research was funded by the Ministry of Science and Technology, Taiwan, grant numbers MOST 108-2314-B-039-009-MY3; the China Medical University, Taiwan, grant number CMU110-MF-31, CMU110-S-43, CMU110-Z-08, CMU109-AWARD-02; and ?Chinese Medicine Research Center, China Medical University? from The Featured Areas Research Center Program within the framework of the Higher Education Sprout Project by the Ministry of Education (MOE) in Taiwan (CMRC-CHM-2-1).Experiments and data analysis were performed in part through the use of the Medical Research Core Facilities Center, Office of Research & Development at China Medical University (Taichung, Taiwan). Publisher Copyright: {\textcopyright} 2022 by the authors. Licensee MDPI, Basel, Switzerland.",
year = "2022",
month = apr,
doi = "10.3390/ijms23084099",
language = "English",
volume = "23",
journal = "International Journal of Molecular Sciences",
issn = "1661-6596",
publisher = "MDPI AG",
number = "8",
}