摘要
We investigated the synergism of colistin and imipenem against a multidrug-resistant K. pneumoniae isolate which was recovered from a severe hip infection. PCR and DNA sequencing were used to characterize the outer membrane porin genes and the resistance genes mediating the common β-lactamases and carbapenemases. Synergism was evaluated by time-kill studies. The b l a SHV-31, b l a CMY-2, and b l a DHA-1 were detected. Outer membrane porin genes analysis revealed loss of ompK36 and frame-shift mutation of ompK35. The common carbapenemase genes were not found. Time-kill studies demonstrated that a combination of 1x MIC of colistin (2 mg/L) and 1x MIC of imipenem (8 mg/L) was synergistic and bactericidal but with inoculum effect. Bactericidal activity without inoculum effect was observed by concentration of 2x MIC of colistin alone or plus 2x MIC of imipenem. In conclusion, colistin plus imipenem could be an alternative option to treat carbapenem-resistant K. pneumoniae infections.
原文 | 英語 |
---|---|
文章編號 | 568079 |
期刊 | BioMed Research International |
卷 | 2015 |
DOIs | |
出版狀態 | 已發佈 - 2015 |
ASJC Scopus subject areas
- 一般免疫學和微生物學
- 一般生物化學,遺傳學和分子生物學