TY - JOUR
T1 - Improvement in resolution of laser capture microdissection using near-field probe to capture nanoparticles
AU - Chen, Chien Ming
AU - Lee, Jen Ai
AU - Yen, Chi Fu
PY - 2009/6
Y1 - 2009/6
N2 - Purpose: A modified laser capture microdissection (LCM) system is developed to improve resolution to 400 nm, using a laser light (808 nm) transmitted by a near-field tip probe. Materials and methods: Using a 150-nm aperture to heat an ethylene vinyl acetate (EVA) film, melted spots on the average of 400 nm in diameter are generated on the underlying target composed of a 20-nm gold-particle monolayer. The near-field tip probe composed of fiber is set on a 2-D nanometer piezoactuator (PZT) for precise capturing of the monolayer of gold particles.The monolayer of gold particles under the target is bound to the EVA film using a laser, while the remaining EVA film stays on the monolayer. Results: The diameter of the melted spots as small as 400 nm are produced and details are provided that demonstrate the feasibility of the nanooperation of this new LCMsystem. Conclusion: The new LCMsystem successfully captures nanoparticles and improves resolution of microdissection to 400 nm. With this LCM system, the isolation of a single organelle or bacterium is possible.
AB - Purpose: A modified laser capture microdissection (LCM) system is developed to improve resolution to 400 nm, using a laser light (808 nm) transmitted by a near-field tip probe. Materials and methods: Using a 150-nm aperture to heat an ethylene vinyl acetate (EVA) film, melted spots on the average of 400 nm in diameter are generated on the underlying target composed of a 20-nm gold-particle monolayer. The near-field tip probe composed of fiber is set on a 2-D nanometer piezoactuator (PZT) for precise capturing of the monolayer of gold particles.The monolayer of gold particles under the target is bound to the EVA film using a laser, while the remaining EVA film stays on the monolayer. Results: The diameter of the melted spots as small as 400 nm are produced and details are provided that demonstrate the feasibility of the nanooperation of this new LCMsystem. Conclusion: The new LCMsystem successfully captures nanoparticles and improves resolution of microdissection to 400 nm. With this LCM system, the isolation of a single organelle or bacterium is possible.
KW - Laser capturemicrodissection (LCM)
KW - Nanooperation
KW - Near-field fiber probe
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U2 - 10.1109/TNB.2009.2019698
DO - 10.1109/TNB.2009.2019698
M3 - Article
C2 - 19336290
AN - SCOPUS:68949094508
SN - 1536-1241
VL - 8
SP - 113
EP - 119
JO - IEEE Transactions on Nanobioscience
JF - IEEE Transactions on Nanobioscience
IS - 2
M1 - 4806083
ER -