TY - JOUR
T1 - Impairment of gingival fibroblast adherence by IL-6/sIL-6R
AU - Naruishi, K.
AU - Takashiba, S.
AU - Nishimura, F.
AU - Chou, H. H.
AU - Arai, H.
AU - Yamada, H.
AU - Murayama, Y.
PY - 2001
Y1 - 2001
N2 - Interleukin-6 (IL-6) binds to human gingival fibroblasts (HGF) in the presence of a soluble form of IL-6 receptor (sIL-6R). We investigated the effects of IL-6 on the functions of HGF in the presence of sIL-6R. HGF changed their morphology from spindle-shaped to round, and detached from the culture dish by stimulation with IL-6/sIL-6R. In this condition, a signal transducer gp130 and a transcription factor Stat3 were phosphorylated, resulting in activation of transcription factors Stat3 and C/EBPβ. Cytoskeletal β-actin and adhesion molecule integrin-α5, a subunit of α5β1 integrin (VLA-5), were found to possess potential binding domains for these transcription factors in their promoters. Accumulation of β-actin and integrin-α5 mRNA decreased, contrary to the expectation of the induction of gene transcription. Furthermore, the decrease in their mRNAs was associated with reduced expression of both actin and VLA-5 proteins. These results suggest that the expression of VLA-5 and actin was down-regulated in HGF through an IL-6 signaling pathway, resulting in impairment of HGF adherence.
AB - Interleukin-6 (IL-6) binds to human gingival fibroblasts (HGF) in the presence of a soluble form of IL-6 receptor (sIL-6R). We investigated the effects of IL-6 on the functions of HGF in the presence of sIL-6R. HGF changed their morphology from spindle-shaped to round, and detached from the culture dish by stimulation with IL-6/sIL-6R. In this condition, a signal transducer gp130 and a transcription factor Stat3 were phosphorylated, resulting in activation of transcription factors Stat3 and C/EBPβ. Cytoskeletal β-actin and adhesion molecule integrin-α5, a subunit of α5β1 integrin (VLA-5), were found to possess potential binding domains for these transcription factors in their promoters. Accumulation of β-actin and integrin-α5 mRNA decreased, contrary to the expectation of the induction of gene transcription. Furthermore, the decrease in their mRNAs was associated with reduced expression of both actin and VLA-5 proteins. These results suggest that the expression of VLA-5 and actin was down-regulated in HGF through an IL-6 signaling pathway, resulting in impairment of HGF adherence.
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U2 - 10.1177/00220345010800050701
DO - 10.1177/00220345010800050701
M3 - Article
C2 - 11437212
AN - SCOPUS:0034955708
SN - 0022-0345
VL - 80
SP - 1421
EP - 1424
JO - Journal of Dental Research
JF - Journal of Dental Research
IS - 5
ER -