TY - JOUR
T1 - IL-5 inhibits apoptosis by upregulation of c-myc expression in human hematopoietic cells
AU - Juan, Shu Hui
AU - Yen, Jeffrey Jong Young
AU - Lee, Horng Mo
AU - Huang, Huei Mei
PY - 2005
Y1 - 2005
N2 - Interleukin 5 (IL-5) inhibition of apoptosis is required throughout many hematopoietic lineages to regulate proliferation and differentiation. It is not clear how IL-5 mediates the intracellular molecular events regulating the anti-apoptotic effect. The cell lines TF-1 and JYTF-1 expressed different amounts of the IL-5 receptor α (IL-5Rα) subunit, which caused contrasting effects in response to IL-5. IL-5 supported the survival but not the anti-apoptotic activities of TF-1 cells, which have a low expression of IL-5Rα. In contrast, IL-5 supported both the survival and the anti-apoptotic activities of JYTF-1 cells, which overexpressed IL-5Rα compared to TF-1 cells. In this study, IL-5 stimulation increased Annexin V binding (indicative of apoptosis) in TF-1 cells and decreased apoptosis in JYTF-1 cells. The proto-oncogenes c-fos, fosB, and c-jun were not detected, whereas junB was induced by IL-5 stimulation in both types of cells. Most importantly, IL-5 significantly induced c-myc expression in JYTF-1 cells, but did not in TF-1 cells. These results are consistent with the possibility that IL-5 inhibits apoptosis in JYTF-1 cells via the upregulation of c-myc expression.
AB - Interleukin 5 (IL-5) inhibition of apoptosis is required throughout many hematopoietic lineages to regulate proliferation and differentiation. It is not clear how IL-5 mediates the intracellular molecular events regulating the anti-apoptotic effect. The cell lines TF-1 and JYTF-1 expressed different amounts of the IL-5 receptor α (IL-5Rα) subunit, which caused contrasting effects in response to IL-5. IL-5 supported the survival but not the anti-apoptotic activities of TF-1 cells, which have a low expression of IL-5Rα. In contrast, IL-5 supported both the survival and the anti-apoptotic activities of JYTF-1 cells, which overexpressed IL-5Rα compared to TF-1 cells. In this study, IL-5 stimulation increased Annexin V binding (indicative of apoptosis) in TF-1 cells and decreased apoptosis in JYTF-1 cells. The proto-oncogenes c-fos, fosB, and c-jun were not detected, whereas junB was induced by IL-5 stimulation in both types of cells. Most importantly, IL-5 significantly induced c-myc expression in JYTF-1 cells, but did not in TF-1 cells. These results are consistent with the possibility that IL-5 inhibits apoptosis in JYTF-1 cells via the upregulation of c-myc expression.
KW - Anti-apoptosis
KW - IL-5 receptor α subunit
KW - Interleukin 5
KW - c-myc
UR - http://www.scopus.com/inward/record.url?scp=22044435018&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=22044435018&partnerID=8YFLogxK
U2 - 10.1196/annals.1338.041
DO - 10.1196/annals.1338.041
M3 - Article
C2 - 15965094
AN - SCOPUS:22044435018
SN - 0077-8923
VL - 1042
SP - 481
EP - 487
JO - Annals of the New York Academy of Sciences
JF - Annals of the New York Academy of Sciences
ER -