Identification of a novel cephalosporinase (DHA-3) in Klebsiella pneumoniae isolated in Taiwan

L. T. Wu, S. W. Hung, Y. C. Chuang, H. E. Chen, R. N. Jones, W. Leon Yu

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9 引文 斯高帕斯(Scopus)


A strain of Klebsiella pneumoniae resistant to cefoxitin and oxyimino-cephalosporins, but susceptible to cefepime, was isolated from an adult patient hospitalised in Taichung, Taiwan. Isoelectric focusing revealed three β-lactamases with isoelectric points of 5.4, 8.2 and 7.9, respectively. Following PCR with plasmid DNA templates and gene sequencing, these enzymes were shown to correspond to TEM-1, SHV-5 and a novel DHA-1-like enzyme (designated DHA-3). The bla genes for TEM-1 and SHV-5 were transferable, but the bla DHA-3 gene was non-self-transferable in conjugation experiments. All three bla genes were successfully introduced by electrotransformation into an Escherichia coli recipient (DH5α), resulting in a similar resistance profile to that observed in the original donor strain. Other K. pneumoniae strains producing DHA-1-like enzymes have been identified previously in Taiwan, and this report suggests that DHA-type β-lactamases are continuing to emerge in this country.

頁(從 - 到)893-897
期刊Clinical Microbiology and Infection
出版狀態已發佈 - 11月 2005

ASJC Scopus subject areas

  • 微生物學(醫學)
  • 微生物學


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