Hyperthermia reduces cancer cell invasion and combats chemoresistance and immune evasion in human bladder cancer

Te Fu Tsai, Thomas I.Sheng Hwang, Po Chun Chen, Yen Chen Chen, Kuang Yu Chou, Chao Yen Ho, Hung En Chen, An Chen Chang

研究成果: 雜誌貢獻文章同行評審

摘要

Bladder cancer (BC) is a common malignancy and its most prevalent type is urothelial carcinoma, which accounts for ~90% of all cases of BC. The current treatment options for BC are limited, which necessitates the development of alterna‑ tive treatment strategies. Hyperthermia (HT), as an adjuvant cancer therapy, is known to improve the efficacy of chemo‑ therapy or radiotherapy. The present study aimed to investigate the anti‑tumor effects of HT on cell survival, invasiveness, chemoresistance and immune evasion in human BC cell lines (5637, T24 and UMUC3). Calcein AM staining was performed to analyze the cytotoxicity of natural killer (NK) cells against human BC cells following HT treatment. Cell migration and invasion affected by HT were analyzed using Transwell migra‑ tion and invasion assays. It was found that HT inhibited the proliferation of BC cells by downregulating the phosphoryla‑ tion of protein kinase B. Moreover, HT effectively enhanced the sensitivity of BC cells to the chemotherapy drug cisplatin (DDP) and reduced the chemoresistance of DDP‑resistant cells by downregulating the expression of cadherin‑11. It was further demonstrated that HT inhibited the migration and invasion of BC cells and enhanced the cytotoxic effects of NK cells. In summary, the antineoplastic effects of HT were medi‑ ated through three main mechanisms: Enhancement of the chemosensitivity of BC cells and mitigation of DDP‑induced chemoresistance, suppression of the invasive potential of BC cells and reinforcement of the anticancer response of NK cells. Thus, HT appears to be a promising adjunctive therapy for human BC.
原文英語
文章編號116
期刊International Journal of Oncology
65
發行號6
DOIs
出版狀態已發佈 - 12月 2024

ASJC Scopus subject areas

  • 腫瘤科
  • 癌症研究

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