Hypermethylation of the p16 gene promoter in ptergia and its association with the expression of DNA methyltransferase 3b

Pei Liang Chen, Ya Wen Cheng, Chun Chi Chiang, Sung Huei Tseng, Pak Sam Chau, Yi Yu Tsai

研究成果: 雜誌貢獻文章同行評審

26 引文 斯高帕斯(Scopus)

摘要

Purpose: A pterygium has long been considered as a degenerative condition. After p53 protein was found to be abnormally expressed in the epithelium, researchers suggested that a pterygium may be a tumor, but additional evidence is required to support this hypothesis. Aberrant methylation of the p16 gene (CDKN2A) promoter and resultant gene silencing play important roles in the pathogenesis of many types of human cancers. The purpose of this study was to investigate hypermethylation of the p16 promoter in pterygia and the relationship between this hypermethylation and the expression of p16 and DNA methyltransferase 3b (DNMT3b) proteins. Methods: We studied the methylation status of p16 and the expression of p16 and DNMT3b proteins by performing methylation-specific polymerase chain reaction and immunohistochemistry, respectively, in specimens of 129 pterygia and 16 normal conjunctiva. The results were statistically analyzed. Results: Hypermethylation of the p16 gene promoter was detected in 21 (16.3%) of 129 pterygial specimens. Among them, 46 (35.7%) were positive for p16 protein expression, and 83 (64.3%) were negative. Staining for p16 was limited to the nuclei of the epithelial layer. We observed a significant reverse correlation between hypermethylation of the p16 promoter and the expression of p16 protein (p=0.006). Thirty-eight (29.5%) pterygial specimens were positive for DNMT3b protein expression, and 91 (70.5%) were negative. DNMT3b staining was limited to the nuclei of the epithelia] layer. A significant correlation was found between hypermethylation of the p16 promoter and the expression of DNMT3b protein (P<0.001). Conclusions: The p16 gene promoter was hypermethylated in pterygia, and this hypermethylation was strongly linked to expression of the positive expression of DNMT3b protein and to the suppression of p16 protein. These data provided molecular evidence that methylation occurs in pterygia and that it may play a role in the their development. © 2006 Molecular Vision.
原文英語
頁(從 - 到)1411-1416
頁數6
期刊Molecular Vision
12
出版狀態已發佈 - 11月 17 2006
對外發佈

ASJC Scopus subject areas

  • 眼科

指紋

深入研究「Hypermethylation of the p16 gene promoter in ptergia and its association with the expression of DNA methyltransferase 3b」主題。共同形成了獨特的指紋。

引用此