Hydraulic extraction of high quality sperms from a dual gradient sperm sorter for in-vitro fertilizatio

Yueh Jen Chen; Ren Guei Wu; Peng Chun Chen; Yu Nan Lin; Huei Wen Hsiu; Ching Chu Yen; Chia-Woei Wang; Li-Chern Pan; Fan Gang Tseng

doi:10.1109/NEMS.2016.7758215

Hydraulic extraction of high quality sperms from a dual gradient sperm sorter for in-vitro fertilizatio

Yueh Jen Chen, Ren Guei Wu, Peng Chun Chen, Yu Nan Lin, Huei Wen Hsiu, Ching Chu Yen, Chia-Woei Wang, Li-Chern Pan, Fan Gang Tseng

研究成果: 書貢獻/報告類型 › 會議貢獻

摘要

This paper proposes a selection and extraction strategy to acquire high quality sperms from a biomimetic microfluidic device capable of generating a dual gradient flow field for motile sperm sorting. The sorter consists of a straight flow zone, a rapidly expansion sector for sperm sorting by gradient up-stream flow, and a dumbbell channel for dead sperms separation at the outlet. A collateral runner is set at the front area of the expansion sector, but mostly kept in dry state without affecting the sorting process. At the end of sorting, a pressure was applied from both the inlet and outlet to flush the high quality sperms into the collateral runner for collection. Controllable numbers, ranging from 1 to 15000, of sperms can be collected with a motility (>60μm) ratio higher than 60%, suitable for in-vitro fertilization (IVF).

原文	英語
主出版物標題	2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016
發行者	Institute of Electrical and Electronics Engineers Inc.
頁面	130-133
頁數	4
ISBN（電子）	9781509019472
DOIs	https://doi.org/10.1109/NEMS.2016.7758215
出版狀態	已發佈 - 11月 28 2016
事件	11th IEEE Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016 - Sendai, 日本持續時間: 4月 17 2016 → 4月 20 2016

出版系列

名字	2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016

其他

其他	11th IEEE Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016
國家/地區	日本
城市	Sendai
期間	4/17/16 → 4/20/16

存取文件

10.1109/NEMS.2016.7758215

其它文件與鏈接

引用此

Chen, Y. J., Wu, R. G., Chen, P. C., Lin, Y. N., Hsiu, H. W., Yen, C. C., Wang, C-W., Pan, L-C., & Tseng, F. G. (2016). Hydraulic extraction of high quality sperms from a dual gradient sperm sorter for in-vitro fertilizatio. 於 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016 (頁 130-133). [7758215] (2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/NEMS.2016.7758215

Hydraulic extraction of high quality sperms from a dual gradient sperm sorter for in-vitro fertilizatio. / Chen, Yueh Jen; Wu, Ren Guei; Chen, Peng Chun 等.
2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016. Institute of Electrical and Electronics Engineers Inc., 2016. p. 130-133 7758215 (2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016).

研究成果: 書貢獻/報告類型 › 會議貢獻

Chen, YJ, Wu, RG, Chen, PC, Lin, YN, Hsiu, HW, Yen, CC, Wang, C-W, Pan, L-C & Tseng, FG 2016, Hydraulic extraction of high quality sperms from a dual gradient sperm sorter for in-vitro fertilizatio. 於 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016., 7758215, 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016, Institute of Electrical and Electronics Engineers Inc., 頁 130-133, 11th IEEE Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016, Sendai, 日本, 4/17/16. https://doi.org/10.1109/NEMS.2016.7758215

Chen YJ, Wu RG, Chen PC, Lin YN, Hsiu HW, Yen CC 等. Hydraulic extraction of high quality sperms from a dual gradient sperm sorter for in-vitro fertilizatio. 於 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016. Institute of Electrical and Electronics Engineers Inc. 2016. p. 130-133. 7758215. (2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016). doi: 10.1109/NEMS.2016.7758215

Chen, Yueh Jen ; Wu, Ren Guei ; Chen, Peng Chun 等. / Hydraulic extraction of high quality sperms from a dual gradient sperm sorter for in-vitro fertilizatio. 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016. Institute of Electrical and Electronics Engineers Inc., 2016. 頁 130-133 (2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016).

@inproceedings{1aa6ad1df0ac454f99344bb369ddabee,

title = "Hydraulic extraction of high quality sperms from a dual gradient sperm sorter for in-vitro fertilizatio",

abstract = "This paper proposes a selection and extraction strategy to acquire high quality sperms from a biomimetic microfluidic device capable of generating a dual gradient flow field for motile sperm sorting. The sorter consists of a straight flow zone, a rapidly expansion sector for sperm sorting by gradient up-stream flow, and a dumbbell channel for dead sperms separation at the outlet. A collateral runner is set at the front area of the expansion sector, but mostly kept in dry state without affecting the sorting process. At the end of sorting, a pressure was applied from both the inlet and outlet to flush the high quality sperms into the collateral runner for collection. Controllable numbers, ranging from 1 to 15000, of sperms can be collected with a motility (>60μm) ratio higher than 60%, suitable for in-vitro fertilization (IVF).",

keywords = "CASA, Chip, PDMS, Sperm",

author = "Chen, {Yueh Jen} and Wu, {Ren Guei} and Chen, {Peng Chun} and Lin, {Yu Nan} and Hsiu, {Huei Wen} and Yen, {Ching Chu} and Chia-Woei Wang and Li-Chern Pan and Tseng, {Fan Gang}",

note = "Funding Information: The National Science Council (Project No. 103- 2321-B-007-004) is gratefully acknowledged. Miss I. L. LIN in the Taipei Medical University provided invaluable assistance on operation of the computer Assisted sperm analyzer.; 11th IEEE Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016 ; Conference date: 17-04-2016 Through 20-04-2016",

year = "2016",

month = nov,

day = "28",

doi = "10.1109/NEMS.2016.7758215",

language = "English",

series = "2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

pages = "130--133",

booktitle = "2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016",

}

TY - GEN

T1 - Hydraulic extraction of high quality sperms from a dual gradient sperm sorter for in-vitro fertilizatio

AU - Chen, Yueh Jen

AU - Wu, Ren Guei

AU - Chen, Peng Chun

AU - Lin, Yu Nan

AU - Hsiu, Huei Wen

AU - Yen, Ching Chu

AU - Wang, Chia-Woei

AU - Pan, Li-Chern

AU - Tseng, Fan Gang

N1 - Funding Information: The National Science Council (Project No. 103- 2321-B-007-004) is gratefully acknowledged. Miss I. L. LIN in the Taipei Medical University provided invaluable assistance on operation of the computer Assisted sperm analyzer.

PY - 2016/11/28

Y1 - 2016/11/28

N2 - This paper proposes a selection and extraction strategy to acquire high quality sperms from a biomimetic microfluidic device capable of generating a dual gradient flow field for motile sperm sorting. The sorter consists of a straight flow zone, a rapidly expansion sector for sperm sorting by gradient up-stream flow, and a dumbbell channel for dead sperms separation at the outlet. A collateral runner is set at the front area of the expansion sector, but mostly kept in dry state without affecting the sorting process. At the end of sorting, a pressure was applied from both the inlet and outlet to flush the high quality sperms into the collateral runner for collection. Controllable numbers, ranging from 1 to 15000, of sperms can be collected with a motility (>60μm) ratio higher than 60%, suitable for in-vitro fertilization (IVF).

AB - This paper proposes a selection and extraction strategy to acquire high quality sperms from a biomimetic microfluidic device capable of generating a dual gradient flow field for motile sperm sorting. The sorter consists of a straight flow zone, a rapidly expansion sector for sperm sorting by gradient up-stream flow, and a dumbbell channel for dead sperms separation at the outlet. A collateral runner is set at the front area of the expansion sector, but mostly kept in dry state without affecting the sorting process. At the end of sorting, a pressure was applied from both the inlet and outlet to flush the high quality sperms into the collateral runner for collection. Controllable numbers, ranging from 1 to 15000, of sperms can be collected with a motility (>60μm) ratio higher than 60%, suitable for in-vitro fertilization (IVF).

KW - CASA

KW - Chip

KW - PDMS

KW - Sperm

UR - http://www.scopus.com/inward/record.url?scp=85007196090&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85007196090&partnerID=8YFLogxK

U2 - 10.1109/NEMS.2016.7758215

DO - 10.1109/NEMS.2016.7758215

M3 - Conference contribution

AN - SCOPUS:85007196090

T3 - 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016

SP - 130

EP - 133

BT - 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016

PB - Institute of Electrical and Electronics Engineers Inc.

T2 - 11th IEEE Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016

Y2 - 17 April 2016 through 20 April 2016

ER -