TY - JOUR
T1 - HUMAN β‐ENDORPHIN
T2 - SYNTHESIS AND CHARACTERIZATION OF ANALOGS IODINATED AND TRITIATED AT TRYOSINE RESIDUES 1 AND 27
AU - HOUGHTEN, RICHARD A.
AU - CHANG, WEN‐CHANG ‐C
AU - LI, CHOH HAO
PY - 1980/1/1
Y1 - 1980/1/1
N2 - Three tritiated analogs of human β‐endorphin have been prepared from their corresponding iodinated analogs. The iodinated analogs (diiodotyrosine at positions 1, 27 or 1 and 27) were synthesized by the solid‐phase method and were found to have biological and physical properties which were altered when compared with the native molecule. Catalytic exchange of these iodinated analogs in the presence of tritium yielded tritiated human β‐endorphins having full biological activity and specific activity of 50–100 Ci/mmol. Both the iodinated and tritiated β‐endorphin analogs were shown to be homogeneous by chromatography on carboxymethylcellulose, partition chromatography, paper chromatography, amino acid analysis, electrophoresis, high performance liquid chromatography, and isoelectric focusing on polyacrylamide.
AB - Three tritiated analogs of human β‐endorphin have been prepared from their corresponding iodinated analogs. The iodinated analogs (diiodotyrosine at positions 1, 27 or 1 and 27) were synthesized by the solid‐phase method and were found to have biological and physical properties which were altered when compared with the native molecule. Catalytic exchange of these iodinated analogs in the presence of tritium yielded tritiated human β‐endorphins having full biological activity and specific activity of 50–100 Ci/mmol. Both the iodinated and tritiated β‐endorphin analogs were shown to be homogeneous by chromatography on carboxymethylcellulose, partition chromatography, paper chromatography, amino acid analysis, electrophoresis, high performance liquid chromatography, and isoelectric focusing on polyacrylamide.
KW - guinea pig ileum assay
KW - iodinated β‐endorphin
KW - solid phase peptide synthesis
KW - tritiated β‐endorphin
UR - http://www.scopus.com/inward/record.url?scp=0019257461&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0019257461&partnerID=8YFLogxK
U2 - 10.1111/j.1399-3011.1980.tb02592.x
DO - 10.1111/j.1399-3011.1980.tb02592.x
M3 - Article
C2 - 7461911
AN - SCOPUS:0019257461
SN - 0367-8377
VL - 16
SP - 311
EP - 320
JO - International journal of protein research
JF - International journal of protein research
IS - 4
ER -