Human haptoglobin phenotypes and concentration determination by nanogold-enhanced electrochemical impedance spectroscopy

Tsai Mu Cheng, Tzu Cheng Lee, Shin Hua Tseng, Hsueh Liang Chu, Ju Pin Pan, Chia Ching Chang

研究成果: 雜誌貢獻文章同行評審

13 引文 斯高帕斯(Scopus)

摘要

Haptoglobin (Hp) is an acute phase protein that binds free hemoglobin (Hb), preventing Hb-induced oxidative damage in the vascular system. There are three phenotypes in human Hp, whose heterogeneous polymorphic structures and varying concentrations in plasma have been attributed to the cause of diseases and outcome of clinical treatments. Different phenotypes of Hp may be composed of the same subunits but different copy numbers, rendering their determination difficult by a single procedure. In this study, we have developed a simple, fast, reliable and sensitive method, using label-free nanogold-modified bioprobes coupled with self-development electrochemical impedance spectroscopy (EIS). By this method, probe surface charge transfer resistance is detected. The relative charge transfer resistance ratios for Hp 1-1, Hp 2-1 and Hp 2-2 were characterized. We were able to determine protein size difference within 3nm, and the linear region of the calibration curve for Hp levels in the range of 90pgml- 1 and 90νgml- 1 (∼1fM to 1pM). We surmise that similar approaches can be used to investigate protein polymorphism and altered protein-protein interaction associated with diseases.
原文英語
文章編號245105
期刊Nanotechnology
22
發行號24
DOIs
出版狀態已發佈 - 6月 17 2011
對外發佈

ASJC Scopus subject areas

  • 生物工程
  • 化學 (全部)
  • 電氣與電子工程
  • 機械工業
  • 材料力學
  • 材料科學(全部)

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