@article{42059184c20a4a5898f6a0e782dfbe1a,
title = "Human adipose-derived mesenchymal stem cells-incorporated silk fibroin as a potential bio-scaffold in guiding bone regeneration",
abstract = "Recently, stem cell-based bone tissue engineering (BTE) has been recognized as a preferable and clinically significant strategy for bone repair. In this study, a pure 3D silk fibroin (SF) scaffold was fabricated as a BTE material using a lyophilization method. We aimed to investigate the efficacy of the SF scaffold with and without seeded human adipose-derived mesenchymal stem cells (hASCs) in facilitating bone regeneration. The effectiveness of the SF-hASCs scaffold was evaluated based on physical characterization, biocompatibility, osteogenic differentiation in vitro, and bone regeneration in critical rat calvarial defects in vivo. The SF scaffold demonstrated superior biocompatibility and significantly promoted osteogenic differentiation of hASCs in vitro. At six and twelve weeks postimplantation, micro-CT showed no statistical difference in new bone formation amongst all groups. However, histological staining results revealed that the SF-hASCs scaffold exhibited a better bone extracellular matrix deposition in the defect regions compared to other groups. Immunohistochemical staining confirmed this result; expression of osteoblast-related genes (BMP-2, COL1a1, and OCN) with the SF-hASCs scaffold treatment was remarkably positive, indicating their ability to achieve effective bone remodeling. Thus, these findings demonstrate that SF can serve as a potential carrier for stem cells, to be used as an osteoconductive bioscaffold for BTE applications",
keywords = "Adipose stem cells, Bone regeneration, Bone tissue engineering, Calvarial defects, Scaffold, Silk fibroin",
author = "Dewi Sartika and Wang, {Chih Hsin} and Wang, {Ding Han} and Cherng, {Juin Hong} and Chang, {Shu Jen} and Fan, {Gang Yi} and Wang, {Yi Wen} and Lee, {Chian Her} and Hong, {Po Da} and Wang, {Chih Chien}",
note = "Funding Information: This work was supported by the Ministry of Science and Technology (MOST 107-2314-B-038-088, MOST 108-2314-B-038-082); the Ministry of National Defense-Medical Affairs Bureau (MAB-109-044, MAB-109-058); Tri-Service General Hospital program (TSGH-C107-081, TSGH-C108-154, TSGH-C108-113, TSGH-D-109141, TSGH-D-10902); Cheng-Hsin General Hospital, and the National Defense Medical Center cooperation (CH-NDMC-108-21). The authors thank Yao-Wen Hong, Cheng-Che Liu, andWen-Zhi Lin from the Institute of Preventive Medicine, National Defense Medical Center, Taipei, Taiwan (R.O.C.) for their assistance with the SEM analysis of characterized polymers. Funding Information: Author Contributions: Conceptualization, D.-H. W. and P.-D. H.; data curation, D. S. and S.-J. C.; formal asnuaplyesrivsi,sCio.-nH, J..W-H..;C fu. annddinCg.a-Ccq.Wui.s;iwtiornit,inCg.-—Ho. rWig.inanaldd Cra.-fCt,.DW.S.;. iannvdesSt.i-gJa.Cti.o;nw,rJi.t-iHn.gC—.;r erevsioeuwrcaensd, Ged.-iYti.nFg.,, YJ.-.-HW.C. . W., and C.-H. L.; supervision, J.-H. C. and C.-C. W.; writing—original draft, D. S. and S.-J. C.; writing—review Funding: This work was supported by the Ministry of Science and Technology (MOST 107-2314-B-038-088, MOST 108-2314-B-038-082); the Ministry of National Defense-Medical Affairs Bureau (MAB-109-044, MAB-109-058); Funding: This work was supported by the Ministry of Science and Technology (MOST 107-2314-B-038-088, MOST 108-2314-B-038-082); the Ministry of National Defense-Medical Affairs Bureau (MAB-109-044, MAB-109-0(5C8H);-NTrDi-MSeCrv-1ic0e8 -2G1e)n. eral Hospital program (TSGH-C107-081, TSGH-C108-154, TSGH-C108-113, TSGH-D-109141, TSGH-D-10902); Cheng-Hsin General Hospital, and the National Defense Medical Center cooperation (CH-NDMC-108-21). Publisher Copyright: {\textcopyright} 2020 by the authors.",
year = "2020",
month = apr,
day = "1",
doi = "10.3390/POLYM12040853",
language = "English",
volume = "12",
journal = "Polymers",
issn = "2073-4360",
publisher = "MDPI AG",
number = "4",
}