@article{6319d181ca6148c7bbafceca98722803,
title = "Highly efficient intracellular protein delivery by cationic polyethyleneimine-modified gelatin nanoparticles",
abstract = "Intracellular protein delivery may provide a safe and non-genome integrated strategy for targeting abnormal or specific cells for applications in cell reprogramming therapy. Thus, highly efficient intracellular functional protein delivery would be beneficial for protein drug discovery. In this study, we generated a cationic polyethyleneimine (PEI)-modified gelatin nanoparticle and evaluated its intracellular protein delivery ability in vitro and in vivo. The experimental results showed that the PEI-modified gelatin nanoparticle had a zeta potential of approximately +60 mV and the particle size was approximately 135 nm. The particle was stable at different biological pH values and temperatures and high protein loading efficiency was observed. The fluorescent image results revealed that large numbers of particles were taken up into the mammalian cells and escaped from the endosomes into the cytoplasm. In a mouse C26 cell-xenograft cancer model, particles accumulated in cancer cells. In conclusion, the PEI-modified gelatin particle may provide a biodegradable and highly efficient protein delivery system for use in regenerative medicine and cancer therapy.",
keywords = "Cationic nanocarrier, Gelatin, Intracellular protein delivery, Nanoparticle, Polyethyleneimine",
author = "Chou, {Ming Ju} and Yu, {Hsing Yi} and Hsia, {Jui Ching} and Chen, {Ying Hou} and Hung, {Tzu Ting} and Chao, {Hsiao Mei} and Edward Chern and Huang, {Yi You}",
note = "Funding Information: We thank the excellent technical assistance of Technology Commons, College of Life Science, National Taiwan University (Taipei, Taiwan). This study was supported by grants from the Ministry of Science and Technology (MOST), Taiwan (NSC: 101-2221-E-002-046-MY3) and the Program Project for Regenerative Medicine (106-3114-B-002-008), MOST, Taiwan.. Funding Information: Acknowledgments: We thank the excellent technical assistance of Technology Commons, College of Life Science, National Taiwan University (Taipei, Taiwan). This study was supported by grants from the Ministry of Science and Technology (MOST), Taiwan (NSC: 101-2221-E-002-046-MY3) and the Program Project for Regenerative Medicine (106-3114-B-002-008), MOST, Taiwan. Publisher Copyright: {\textcopyright} 2018 by the authors.",
year = "2018",
month = feb,
day = "15",
doi = "10.3390/ma11020301",
language = "English",
volume = "11",
journal = "Materials",
issn = "1996-1944",
publisher = "MDPI AG",
number = "2",
}