High throughput and parallel flow cytometer using microball lens integrated 3D microfluidic device

Yu Jui Fan; Yen Ling Weng; Horn Jiunn Sheen

doi:10.1109/NEMS.2017.8017057

High throughput and parallel flow cytometer using microball lens integrated 3D microfluidic device

Yu Jui Fan, Yen Ling Weng, Horn Jiunn Sheen

研究成果: 書貢獻/報告類型 › 會議貢獻

1 引文斯高帕斯（Scopus）

摘要

We report an ultrahigh throughput microflow cytometer which has 32 detection flow channels with 3D flow focusing for each channel. This flow cytometer is realized by integrating a high N.A. microball lens array with a 3D microfluidic device, which allows introducing cell samples into 32 sample channels. There are, 64 channels for sheath flow used for parallel lateral focusing and 32 sounding flows for parallel sounding focusing. All microchannels use only three inlets. Results show that a 95% yield rate by standard 10 μm bead detection has been achieved, and a throughput of 1,400,000 cell/sec has been accomplished on a single device.

原文	英語
主出版物標題	2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017
發行者	Institute of Electrical and Electronics Engineers Inc.
頁面	426-429
頁數	4
ISBN（電子）	9781509030590
DOIs	https://doi.org/10.1109/NEMS.2017.8017057
出版狀態	已發佈 - 8月 25 2017
事件	12th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017 - Los Angeles, 美国持續時間: 4月 9 2017 → 4月 12 2017

會議

會議	12th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017
國家/地區	美国
城市	Los Angeles
期間	4/9/17 → 4/12/17

ASJC Scopus subject areas

電氣與電子工程
機械工業
電子、光磁材料
儀器

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10.1109/NEMS.2017.8017057

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Fan, Y. J., Weng, Y. L., & Sheen, H. J. (2017). High throughput and parallel flow cytometer using microball lens integrated 3D microfluidic device. 於 2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017 (頁 426-429). Article 8017057 Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/NEMS.2017.8017057

High throughput and parallel flow cytometer using microball lens integrated 3D microfluidic device. / Fan, Yu Jui; Weng, Yen Ling; Sheen, Horn Jiunn.
2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017. Institute of Electrical and Electronics Engineers Inc., 2017. p. 426-429 8017057.

研究成果: 書貢獻/報告類型 › 會議貢獻

Fan, YJ, Weng, YL & Sheen, HJ 2017, High throughput and parallel flow cytometer using microball lens integrated 3D microfluidic device. 於 2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017., 8017057, Institute of Electrical and Electronics Engineers Inc., 頁 426-429, 12th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017, Los Angeles, 美国, 4/9/17. https://doi.org/10.1109/NEMS.2017.8017057

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abstract = "We report an ultrahigh throughput microflow cytometer which has 32 detection flow channels with 3D flow focusing for each channel. This flow cytometer is realized by integrating a high N.A. microball lens array with a 3D microfluidic device, which allows introducing cell samples into 32 sample channels. There are, 64 channels for sheath flow used for parallel lateral focusing and 32 sounding flows for parallel sounding focusing. All microchannels use only three inlets. Results show that a 95% yield rate by standard 10 μm bead detection has been achieved, and a throughput of 1,400,000 cell/sec has been accomplished on a single device.",

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author = "Fan, {Yu Jui} and Weng, {Yen Ling} and Sheen, {Horn Jiunn}",

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doi = "10.1109/NEMS.2017.8017057",

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AU - Fan, Yu Jui

AU - Weng, Yen Ling

AU - Sheen, Horn Jiunn

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N2 - We report an ultrahigh throughput microflow cytometer which has 32 detection flow channels with 3D flow focusing for each channel. This flow cytometer is realized by integrating a high N.A. microball lens array with a 3D microfluidic device, which allows introducing cell samples into 32 sample channels. There are, 64 channels for sheath flow used for parallel lateral focusing and 32 sounding flows for parallel sounding focusing. All microchannels use only three inlets. Results show that a 95% yield rate by standard 10 μm bead detection has been achieved, and a throughput of 1,400,000 cell/sec has been accomplished on a single device.

AB - We report an ultrahigh throughput microflow cytometer which has 32 detection flow channels with 3D flow focusing for each channel. This flow cytometer is realized by integrating a high N.A. microball lens array with a 3D microfluidic device, which allows introducing cell samples into 32 sample channels. There are, 64 channels for sheath flow used for parallel lateral focusing and 32 sounding flows for parallel sounding focusing. All microchannels use only three inlets. Results show that a 95% yield rate by standard 10 μm bead detection has been achieved, and a throughput of 1,400,000 cell/sec has been accomplished on a single device.

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KW - microfluidics

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BT - 2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017

PB - Institute of Electrical and Electronics Engineers Inc.

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High throughput and parallel flow cytometer using microball lens integrated 3D microfluidic device

摘要

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ASJC Scopus subject areas

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